| Project/Area Number |
13670738
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Osaka City University |
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Principal Investigator |
TAKEUCHI Kazuhide Osaka City University, Graduate School of Medicine, Professor, 大学院・医学研究科, 教授 (80117952)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIYAMA Minoru Osaka City University, Graduate School of Medicine, Associate Professor, 大学院・医学研究科, 助教授 (30240956)
YOSHIKAWA Junichi Osaka City University, Graduate School of Medicine, Professor, 大学院・医学研究科, 教授 (60275245)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2001: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | Myocardial infarction / Remodeling / Cell therapy / Stem Cell / Heart failure / Signal transduction / Myocardial infarction / Stemcell / 心臓リモデリング / 再生医学 / 冠動脈リモデリング |
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| Research Abstract |
Myocardial infarction (MI) promotes deleterious remodeling of the myocardium, resulting in ventricular dilation and pump dysfunction. We examined whether supplementing infarcted myocardium with stem cell would attenuate deleterious remodeling, and enhance contractile performance. Experimental MI was induced by 24-hours coronary ligation followed by reperfusion in adult male Lewis rats and neonatal myocytes were injected directly into the infarct region. Three groups of animals were studied at 4 weeks after cell therapy: noninfarcted control (control), MI plus sham injection (MI), and MI plus stem cell injection (MI+cell). In vivo cardiac function was assessed by echocardiography. MI and MI+cell hearts had indistinguishable infarct sizes of 30% of the LV. At 4 weeks after cell therapy, 92% (13 of 14) of MI+cell hearts showed evidence of myocytes graft survival. MI+cell hearts exhibited attenuation of global ventricular dilation and reduced septum and free wall diameter compared with MI hearts not receiving cell therapy. Implanted stem cell form viable grafts in infarcted myocardium, resulting in enhanced contractile function and attenuated ventricular dilation. These data illustrate that stem cell implantation after MI improves both in systolic and diastolic dysfunction and deleterious remodeling and suggests that cellular implantation may be beneficial after MI.
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