| Project/Area Number |
13670746
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Circulatory organs internal medicine
|
|---|
| Research Institution | Jikei University School of Medicine |
|---|
Principal Investigator |
SUZUKI Hideaki JIKEI UNIVERSITY SCHOOL OF MEDICINES, Lecturer, 医学部, 講師 (70196856)
|
|---|
| Project Period (FY) |
2001 – 2003
|
| Project Status |
Completed (Fiscal Year 2003)
|
| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2003: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2001: ¥1,300,000 (Direct Cost: ¥1,300,000)
|
|---|
| Keywords | Laminin / Transcription factor / Promoter / Cloning / Hybrid-system / Luciferase / Yeast / Plasmid / クローニング / ハイブリットシステム / ハシフェラーゼ |
|---|
| Research Abstract |
Laminin is a major component of the extracellular. Laminin is known to incease in the myocytes, vasucular smooth muscle cells, and renal cells in diabetes and cariomyopathy.
The laminin 1 chain promoter contains a transcriptional element denoted bcn-1(Suzuki H, Bomsztyk K, et al.J.Biol.Chem.271, 1996). bn-1 is PMA and IL-1 inducible in EMSA. Yeast one hybrid screen of mammalian cDNA libraries was used to identify proteins to responcible for the activity of bcn-1 element. Using this strategy we identified that K46(SOL-1)clone binds to the bcn-1 element. To identify the molecular mechanisms of SOL-1, we used the yeast two-hybrid screen from mouse heart cDNA library and cloned one protein, whuich binds to SOL-1.
We are now checking the interaction of SOL-1 and SMARP to the increase effect of laminin gene promoter activity.
|
