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Viral infection and Cardiomyopathy: enteroviral Replication and Cardiocyte Injury in the Recipient Hearts of Dilated Cardiomyopathy and Perforin Knockout Mice with Viral Myocarditis

Research Project

Project/Area Number 13670757
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Circulatory organs internal medicine
Research InstitutionOsaka Medical College

Principal Investigator

DEGUCHI Hirofumi  Osaka Medical College Faculty of Medicine Assistant Professor, 医学部, 助教授 (90131341)

Co-Investigator(Kenkyū-buntansha) FUJIOKA Shigekazu  Osaka Medical College Faculty of Medicine Research Associate, 医学部, 助手 (20319528)
UKIMURA Akira  Osaka Medical College Faculty of Medicine Research Associate, 医学部, 助手 (50257862)
KITAURA Yasushi  Osaka Medical College Faculty of Medicine Professor, 医学部, 教授 (50084950)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 2002: ¥300,000 (Direct Cost: ¥300,000)
KeywordsDilated cardiomyopathy / Cardiac transplantation / Viral myocarditis / Coxsackievirus / Cytokine / Perforin / Knockout mouse / PCR
Research Abstract

(A) Enteroviral detection and cytokine expression in the recipient hearts from patients with dilated cardiomyopathy (DCM)
We examined myocardial specimens obtained from 30 DCM patients who underwent heart transplantation.
(1) Semiquantitative analysis of histological findings: histological findings showed inflammatory cell infiltration in 14DCM patients (47%) and fibrosis in 18 patients (60%). 4 patients (13%) had active myocarditis.
(2) Cytokine expression in the heart: We elucidated the expression of inflammatory cytokine in the recipient hearts using the ABI PRISM7700. IL-1β mRNA was overexpressed in 5 of 12 DCM patients (42%), and IL-8 in 5 of 12 (42%). Expression of IL-12p35, IL15 and IFN-γ mRNA raised respectively in 1 of 12 patients (8%). The findings suggest IL-1β and IL-8 play significant role in the inflammatory lesion in the myocardium of DCM.
(B) Experimental Coxsackievirus B3 Myocarditis in Perforin Knockout (pfk) Mice
We examined light and electron microscopic (EM) changes of the myocardium obtained from pfk and wild (C57BL/6) mice with Coxsackievirus B3 myocarditis. In wild mice many cardiocytes underwent necrosis with infiltration of lymphocytes and macrophages. By immuno EM perforin were often observed in the granules of lymphocytes. These lymphocytes showed close contact with cardiocytes. In pfk mice cardiocyte necrosis was less than in wild mice, although significant number of lymphocytes infiltrated in the myocardium. These findings suggest that pfk plays an important role in the development of lymphocyte-mediated cardiocyte injury.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Ukimura A, Deguchi H, Kitaura Y et al.: "Quantitative analyasis of cytokine mRNA expression in hearts from patients with non-ischemic dilated cardiomyopathy (DCM)"J Card Surg. 18(in press). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Deguchi H, Fujioka S, Ukimura A, Kitaura Y et al.: "Enterovirus RNA replication in cases of dilated cardiomyopathy Light microsopic in situ hybridization and virological analyses of myocardial specimens"J Card Surg. 16. 64-71 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Fujioka S, Kitaura Y: "Coxsackie B virus infection in idiopathic dilated cardiomyopathy Clinical and pharmacological implications"Bio Drugs. 15. 791-799 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 浮村聡, 出口寛文, 北浦泰 他: "拡張型心筋症患者心筋からのエンテロウイルスゲノム全長の検索"厚生労働省特定疾患特発性心筋症調査研究班 平成14年度報告集. (印刷中). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 北浦泰, 浮村聡, 出口寛文 他: "脇炎と心筋症のかかわりあい"循環器科. 50. 46-52 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 北浦泰, 藤岡重和, 浮村聡, 出口寛文 他: "拡張型心筋症の病因と治療に関する研究(第1報)左室部分切除術における切除心筋を用いた検討"日本内科学会雑誌. 91. 240-240 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 浮村聡, 北浦泰: "エクセルナース感染症編 内科系病棟-循環器科"メデイカルレビュー社. 15 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ukimura A, Terasaki F, Fujioka S, Deguchi H, Kitaura Y, Isomura T, and Suma H: "Quantitative analysis of cytokine mRNA expression in hearts from patients with non-ischemic dilated cardiomyopathy (DCM)"J Card Surg. in press. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Fujioka S, Kitaura Y: "Coxsackie B virus infection in idiopathic dilated cardiomyopathy: Clinical and pharmacological implications"Bio Drugs. 15. 791-799 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ukimura A, Terasaki F, Fujioka S, Deguchi H, Kitaura Y, Isomura T, and Suma H: "The quantitative analysis of cytokine mRNA expression in the myocardium of non-ischemic dilated cardiomyopathy (DCM)"Circ J. vol. 66 sup. 602 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Deguchi H, Fujioka S, Ukimura A, Kitaura Y et al: "Enterovirus RNA replication in cases of dilated cardiomyopathy: Light microscopic in situ hybridization and virological analyses of myocardial specimens obtained at partialleftventriculectomy"J Card Surg. 16. 64-71 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ukimura A, Deguchi H, Kitaura Y et al.: "Quantitative analysis of cytokine mRNA expression in hearts from patients with non-ischemic dilated cardiomyopathy(DCM)"J Card Surg.. 18(in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Deguchi H, Fujioka S, Ukimura A, Kitaura Y et al.: "Enterovirus RNA replication in cases of dilated cardiomyophaty : Light microscopic in situ hybridization and virological analyses of myocardial specimens"J Card Surg.. 16. 64-71 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Fujioka S, Kitaura Y: "Coxsackie B virus infection in idiopathic dilated cardiomyopathy ; clinical and pharmacological implications"Bio Drugs. 15. 791-799 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] 浮村聡, 出口寛文, 北浦 泰 他: "拡張型心筋症患者心筋からのエンテロウイルスウイルスゲノム全長の検索"厚生労働省特定疾患突発性心筋症調査研究班平成14年度報告集. (印刷中). (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 北浦泰, 浮村聡, 出口寛文 他: "心筋炎と心筋症のかかわりあい"循環器科. 50巻1号. 46-52 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] 浮村 聡, 北浦 泰: "エクセルナース[感染症編]内科系病棟-循環器科"メディカルレビュー社. 549 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Deguchi H, Fujioka S.Terasaki F, Ukimura A, Kitaura Y et al.: "Enterovirus RNA replication in cases of dilated cardiomyopathy: Light microscopic in situ hybridization and virological analyses of myocardial specimens obtained at PLV"Journal Cardiac Surgery. 16. 64-71 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Fujioka S, Kitaura Y, Ukimura A, Deguchi H et al.: "Evaluation of viral infection in the myocardium of patients with idiopathic dilated cardiomyopathy"Journal of American College Cardiology. 36. 1920-1926 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Fujioka S, Terasaki F, Kitaura Y, Ukimura A, Deguchi H et al.: "CoxsackieB virus RNA replication in the myocardium of patients with end-stage dilated cardiomyopathy"Journal of Molecular Cellular Cardiology. 31. A-181 (1999)

    • Related Report
      2001 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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