| Project/Area Number |
13670774
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Fukuoka Dental College (2002)
Fukuoka College of Health Sciences (2001) |
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Principal Investigator |
NAKAHIMA Yoshiyuki (2002) Fukuoka Dental College, Dentistry, Professor, 歯学部, 教授 (70078749)
笹栗 学 (2001) 福岡医療短期大学, 保健福祉学科, 教授 (00178675)
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| Co-Investigator(Kenkyū-buntansha) |
中島 與志行 福岡歯科大学, 歯学部, 教授 (70078749)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2001: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | Kallikrein / Kinin / cloning / human tissues |
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| Research Abstract |
Abstract
We previously purified a novel kallikrein-like enzyme from the dog heart and demonstrated that it is not only able to form kinins but can also convert angiotensin (Ang) I to Ang II. To clarity whether this novel kallikrein-like enzyme is present in human tissue, we tried to clone this enzyme. In the present study, we have subcloned a kallikrein gene from the human heart and neutrophil cDNA library by using degenerate oligonucleotide and nested PCR amplification. The transcript is identical with the partial sequence of a human renal kallikrein gene. So far, we have not cloned a kallikrein gene from the human heart and neutrophil cDNA library by using this transcript as a target DNA. These results suggest that a tissue kallikrein exerts a variety of biological effects not only in renal tissue, but also human heart and neutrophils. However, it remains unresolved whether this enzyme is novel.
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