• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Cytogenetically analysis of congenital abnormality and childhood malignancy using multicolor FISH methods

Research Project

Project/Area Number 13670808
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pediatrics
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

TASHIRO Satoshi  Hiroshima University, Graduate School of Biomedical Sciences, Assistant Professor, 大学院・医歯薬学総合研究科, 講師 (20243610)

Co-Investigator(Kenkyū-buntansha) IGARASHI Kazuhiko  Hiroshima University, Graduate School of Biomedical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (00250738)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2001: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsColor banding FISH method / childhood leukemia
Research Abstract

We established Color banding FISH method for the precise cytogenetical analysis, and started collection of clinical sample in 2001.
We are testing the ways to transfer clinical sample to Prof. Michael Speeches, a collaborator, for multicolor FISH analysis of these clinical samples in Munich. Clinical samples of childhood leukemia are provided from "Japan Association of Childhood Leukemia Study, J*****.
To identify new genes associated with childhood leukemia by the new method combining color banding FISH method and conventional FISH method to detect a specific gene, we have done functional analysis of some candidate genes. Among them, we have revealed that BACH2, a B cell specific transcription regulator, showed induction of apoptosis upon oxidative stress. Furthermore, we also found that Bach2 is expressed not only in B cell but also in brain.
We have established a method to study the dynamics of chromosome in living cell to study the mechanism of chromosome translocation with Prof. Thomas Cremer, a Collaborator in Munich, and study the dynamics of chromosome territories using method.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Walter J: "Chromosome order in HeLa cells changes during mitosis and early G1, but is stably maintained during subsequent interphase stages"J Cell Biol. 160. 685-697 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Walter J: "A new system for laser-UVA-microirradiation of living cells"J Microsc. 209. 71-75 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sun J: "Hemoprotein Bach1 regulates enhancer availability of heme oxygenase-1 gene"EMBO J.. 21. 5216-5224 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Muto A: "Activation of Maf/AP-1 repressor Bach2 by oxidative stress promotes apoptosis and its interaction with promyelocytic leukemia nuclear bodies"J Biol Chem. 277. 20724-20733 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hoshino, H: "Expression of the oxidative stress-regulated transcription factor Bach2 in differentiating neuronal cells"J. Biochem.. 132. 427-431 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Walter J., Cremer T., Miyagawa K., Tashiro S: "A new system for laser-UVA-microirradiation of living cells"J microsc. 209. 71-75 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Walter J., Schermelleh L., Cremer M., Tashiro S., Cremer T.: "Chromosome order in HeLa cells changes during mitosis and early G1, but is stably maintained during subsequent inerphase stages."J Cell Biol.. 160. 685-697 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sun J., Hoshino H., Takaku K., Nakajima O., Muto A., Suzuki H., Tashiro S., Takahashi S., Shibahara S., Alam J., Taketo MM. Yamamoto M., lgarashi K.: "Hemoprotein Bach1 regulates enhancer availability of heme oxygenase-1 gene"EMBO J.. 21(19). 5126-5224 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Muto A., Tashiro S., Tsuchiya H., Kume A., Kanno M., Ito E., Yamamoto M., Iarashi K.: "Activation of Maf / AP-1 repressor Bach2 by oxidative transcription factor Bach 2 in differentiating neuronal cells"J Biochem. 277(23). 427-431 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hoshino H., Igarashi K.: "Expression of the oxidative stree-regulated transcription factor Bach2 in differentiating neuronal cells"J Blochem. 132. 427-431 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Walter J: "Chromosome order in HeLa cells changes during mitosis and early G1, but is stably maintained during subsequent interphase stages"J Cell Biol. 160. 685-697 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Walter J: "A new system for laser-UVA-microirradiation of living cells"J Microsc. 209. 71-75 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sun J: "Hemoprotein Bach1 regulates enhancer availability of heme oxygenase-1 gene"EMBO J.. 21. 5216-5224 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Muto A: "Activation of Maf/AP-1 repressor Bach2 by oxidative stress promotes apoptosis and its interaction with promyelocytic leukemia nuclear bodies"J Biol Chem. 277. 20724-20733 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hoshino, H: "Expression of the oxidative stress-regulated transcription factor Bach2 in differentiating neuronal cells"J. Biochem.. 132. 427-431 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] katsuoka, F.: "Small Maf compound mutants display central nervous system neuronal degeneration, aberrant transcription, and Bach protein mislocalization coincident with mvoclonus and abnormal startle response"Mol Cell Biol.. 23. 1163-1174 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Miyagawa, K.: "A role for RAD54B in homologous recombination in human cells"EMBO J. 21. 175-180 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kanezaki R.: "Transcription factor BACH1 is recruited to the nucleus by its novel alternative spliced isoform"J. Biol. Chem.. 276. 7278-7284 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ogawa, k: "Heme mediates de-repression of Maf recognition element through direct binding to transcription repressor Bach1"EMBO J.. 20. 2835-2843 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Leach, K.: "Reconstitution of human b-Globin locus control region hypersensitive sites in the absence of chromatin assembly"Mol. Cell. Biol.. 21. 2629-2640 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Sun, J.: "Promoter of Mouse Transcription Repressor bach1 Is Regulated by Sp1 and Trans-Activated by Bach1"J. Biochem.. 130. 385-392 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Sawado, T.: "Activation of b-major globin gene transcription is associated with recruitment of NF-E2 to the b-globin LCR and gene promoter"Proc. Natl. Acad. Sci. U.S.A.. 98. 10226-10231 (2001)

    • Related Report
      2001 Annual Research Report

URL: 

Published: 2001-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi