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Indirect immunofluorescence using anti MLL gene protein

Research Project

Project/Area Number 13670809
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pediatrics
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

UEDA Kazuhiro  Hiroshima University Graduate School of Biomedical Sciences Professor, 大学院・医歯薬学総合研究科, 教授 (30112189)

Co-Investigator(Kenkyū-buntansha) TASHIRO Satoshi  Hiroshima University Graduate School of Biomedical Sciences Assistant Professor, 大学院・医歯薬学総合研究科, 講師 (20243610)
NISHIMURA Shin-ichiro  Hiroshima University Medical Hospital Assistant Professor, 医学部附属病院, 講師 (00228222)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 2002: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2001: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsMLL gene / indirect immunofluorescence / infant leukemia
Research Abstract

First, we prepared poly-clonal antibody to MLL gene protein, but this poly-clonal antibody was not specific for MLL gene protein by Western blotting analysis. So, other anti MLL gene protein antibody (provided by Dr.Seto, Aichi Cancer Institute) was used for further analysis. By immunofluorescence using this antibody, MLL gene and MLL chimeric gene was located at nucleus of leukemic cells. However, we could not detect the difference of distribution between MLL *ne and MLL chimeric gene protein on the nucleus of the leukemic cell. Quantitative analysis of the protein was also difficult by immunohistochemistry using this antibody. New mono-clonal antibody, which detect the difference between MLL gene and MLL chimeric gene protein, should be synthesized.
Although we analyzed few clinical samples, rapid diagnosis of infant leukemia by immunofluorescence using anti MLL gene protein antibody could not be established. For diagnosis of infant leukemia with chromosomal translocations, multiplex PCR analysis may be superior than immunohistochemistry. In the clinical study of childhood acute lymphoblastic leukemia of JACLS : Japan Association of Childhood Leukemia Study, multiplex PCR analysis will be underwent at all patients with denove acute lymphoblastic leukemia.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] H.Kawasaki: "Superior outcome of infant acute myeloid leukemia with intensive chemotherapy : results of the Japan Infant Leukemia Study Group"Blood. 98(13). 3589-3594 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Miyagawa: "A role for RAD54B in homologous recombination in human cells"EMBO J. 21. 175-180 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Isoyama: "Risk-directed treatment of infant acute lymphoblastic leukemia based on early assessment of MLL gene status"Br J Haematol. 118. 999-1010 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sun J: "Hemoprotein Bach1 regulates enhancer availability of heme oxygenase-1 gene"EMBO J.. 21. 5216-5224 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Muto A: "Activation of Maf/AP-1 repressor Bach2 by oxidative stress promotes apoptosis and its interaction with promyelocytic leukemia nuclear bodies"J Biol Chem. 277. 20724-20733 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Kawasaki: "Superior outcome of infant acute myeloid leukemia with intensive chemotherapy : results of the Japan Infant Leukemia Study Group"Blood. 98(13). 3589-3594 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Miyagawa: "A role for RAD54B in homologous recombination in human cells"EMBO J. 21. 175-180 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Isoyama: "Risk-directed treatment of infant acute lymphoblastic leukemia based on early assessment of MLL gene status"Br J Haematol. 118. 999-1010 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sun J: "Hemoprotein Bach1 regulates enhancer availability of heme oxygenase-1 gene"EMBO J. 21. 5216-5224 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Muto A: "Activation of Maf/AP-1 repressor Bach2 by oxidative stress promotes apoptosis and its interaction with promyelocytic leukemia nuclear bodies"J Biol Chem. 277. 20724-20733 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Kawasaki: "Superior outcome of infant acute myeloid leukemia with intensive chemotherapy : results of the Japan Infant Leukemia Study Group"Blood. 98(13). 3589-3594 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] K.Miyagawa: "A role for RAD54B in homologous recombination in human cells"EMBO J. 21. 175-180 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] K.Isoyama: "Risk-directed treatment of infant acute lymphoblastic leukemia based on early assessment of MLL gene status"Br J Haematol. 118. 999-1010 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sun J: "Hemoprotein Bach1 regulates enhancer availability of heme oxygenase-1 gene"EMBO J.. 21. 5216-5224 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Muto A: "Activation of Maf/AP-1 repressor Bach2 by oxidative stress promotes apoptosis and its interaction with promyelocytic leukemia nuclear bodies"J Biol Chem. 277. 20724-20733 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] H.Kawasaki: "Superior outcome of infant acute myeloid leukemia with intensive chemotherapy : results of the Japan Infant Leukemia Study Group"Blood. 98(13). 3589-3594 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kiyoshi Miyagawa: "A role for RAD54B in homologous recombination in human cells"EMBO J. 21. 175-180 (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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