Genetic variability of respiratory syncytial virus isolated during the last 20 years from Sapporo

• Project/Area Number: 13670822
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Sapporo Medical University School of Medicine
• Principal Investigator: TSUTSUMI Hiroyuki Professor, Department of Pediatrics, Sapporo Medical University, School of Medicine, 医学部, 教授 (80217348)
• Project Period (FY): 2001 – 2002
• Project Status: Completed (Fiscal Year 2002)
• Budget Amount: ¥3,300,000 (Direct Cost: ¥3,300,000); Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000); Fiscal Year 2001: ¥2,000,000 (Direct Cost: ¥2,000,000)
• Keywords: RS virus / molecular epidemiology / herd immunity / RSV / PcR-RFLP / RSV G protein

Research Abstract

The genetic variability of 125 respiratory synsytial virus (RSV) subgroup A and 32 subgroup B isolates over 19 epidemics from 1980 to 1999 in Sapporo city was investigated. Allocation of isolates into lineages was archived by RT-PCR of selected regions of large glycoproteins (G) gene followed by RFLP analysis and/or direct sequencing of RT-PCR product. In RSV subgroup A isolates 10 different genetic lineages were identified. The strains with the same genotypes were isolated in each epidemic and the dominant lineages were replaced by others after every one to three consecutive epidemics. However there was no apparent accumulation of diversity with age to indicate progressive changes. The dominant strains were often closely related to those isolated in other parts of the world at a similar time.
Phylogenic analysis of nucleotide sequences of G gene of 32 RSV subgroup B strain revealed seven distinct branches in which strains isoalted during seasons of close proximity were located. There was a tendency for recent isolates to lie at the end of each branch and these linear evolutionary changes were typically represented in a branch containing nine strains isolated during 6 seasons from 1980 to 1986.

Research Products

• [Publications] Seki K, Tsutsumi H, et al.: "Genetic variability of respiratory syncyrial virus 〜"J Med Virol. 64. 374-380 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kawasaki H, Tsutsumi H, et al.: "Genetic variability of respiratory syncyrial 〜"Arch Virol. 146. 457-466 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Seki K, Tsutsumi H, Ohsaki M, et al: "Genetic variability of respiratory syncytial virus subgroup A strain in 15 successive epidemics in one city"J Med Virol. 64. 374-380 (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kamasaki H, Tsutsumi H, Seki K, Chiba S: "Genetic variability of respiratory syncytial virus subgroup B strain isolated during the last 20 years from the same"Arch Virol. 146. 457-466 (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Seki K, Tsutsumi H, Ohsaki M, Kamasaki H, Chiba S.: "Genetic variability of respiratory syncitial virus subgroup A strain in 15 successive Epidemics in one city"J Med Virol. 64. 374-380 (2001)
• [Publications] Kamasaki H, Tsutsumi H, Seki K, Chiba S.: "Genetic variability of respiratory syncytial virus subgroup B strain isolated during The last 20 years from the same region in Japan : existence of time-dependent linear genetic drifts"Arch Virol. 146. 457-466 (2001)
• [Publications] Seki T, Tsutsumi H, Ohsak M, Kamasaki, H, Chiba S.: "Genetic variability of respiratory syncytial virus subgroup A strain in 15 successive epidemics in one city"J. Med. Virol.. 64. 374-380 (2001)