| Project/Area Number |
13670838
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Keio University |
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Principal Investigator |
HASEGAWA Tomonobu Keio University, School of Medicine, Pediatrics, Associate Professor, 医学部, 助教授 (20189533)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2001: ¥2,600,000 (Direct Cost: ¥2,600,000)
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| Keywords | StAE / StAR knockout mice / StAR / GnRH double knockout mice / testes / ovary / lipid deposit / ゴナドトロピン / エストラジオール / 卵胞成熟 / テストステロン / 精子形成 |
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| Research Abstract |
I generated steroidogenic acute regulatory protein (StAR) knockout mice (StAR KO) using gene targeting. I crossed StAR KO mice and naturally occurred gonadotropin-releasiug hormone (GnRH) gene abnormal mice to generate StAR/GnRH double mutant mice (StAR/GnRH double KO). I investigated gonadal histology in StAR KO and StAR/GnRH double KO from birth to 8 weeks.
1. at birth : (1) Testes in StAR KO had mild lipid deposits in interstitial space. Ovaries did not. (2) Both testes and ovaries in StAR/GnRH double KO did not have any Iipid deposits.
2. from birth to 6 weeks : (1) Both testes and ovaries in StAR KO did not have massive lipid deposits. (2) Both testes and ovaries in StAR/GnRH double KO did not have any lipid deposits.
3. 8 weeks : (1) Both testes and ovaries in StAR/GnRH double KO were hypoplastic compared to StAR KO. (2) Both testes and ovaries in StAR KO had massive lipid deposits. (3) Both testes and ovaries in StAR/GnRH double KO lacked lipid deposits. (4) Exogenous gonadotoropin treatment in StAR/GnRH double KO induced lipid deposits in both testes and ovaries.
These data strongly indicated that lipid deposits in StAR KO gonads after pubertal age is gonadotoropin dependent.
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