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The detection of blocking antibody against plasma thrombopoietin in subjects with childhood idiopathic thrombocytopenic purpura

Research Project

Project/Area Number 13670842
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pediatrics
Research InstitutionJIKEI UNIVERSITY SCHOOL OF MEDICINE

Principal Investigator

FUJISAWA Kohji  JIKEI UNIVERSITY SCHOOL OF MEDICINE, DEPARTMENT OF PAlblATRICS, ASSOCIATE PROFESSOR, 医学部, 助教授 (10130197)

Co-Investigator(Kenkyū-buntansha) IYORI Hideki  JIKEI UNIVERSITY SCHOOL OF MEDICINE, DEPARTMENT OF PADIATRICS, ASSISTANT PROFESSOR, 医学部, 助手 (90213256)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2002: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2001: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsITP / autoantibody / TPO / c-mplligand / anti TPO antibody / 抗TPO抗体
Research Abstract

Idiopathic thrombocytopenic purpura (ITP) is a relatively common hermorrhagic disorder characterized by isolated thrombocytopenia without any identifiable systemic disease. Although it is widely believed that the autoantibody-mediated platelet clearance through phagocytic system contributes to thrombocytopenia, the alteration of megakaryocyte maturation and subsequent platelet production is also evidenced indicating "mixed" pathophysiology of ITP. We prebiously reported that marked decrease of TPO-dependent megikaryocytopoiesis of CBMC was noted concomitant with a drop in number of cells morphologically identified as megakaryocytes when cultured in the existence of sera from ITP patients. To further identify pathophysiology of ITP, we developed new ELISA system to detect anti-TPO autoantibodies in patients' sera. Briefly, 96-well plate was coated with polyclonal antibody against TPO followed by adding satulating amount of rhTPO. Sera from ITP patients were then added replicately to wells, and bound IgG antibody was detected by biotin-labeled monoclonal antibody against human IgG and avidin-biotin-peroxidase sy stem. Study protocol was explained and informed consent was obtained for all enrolled. Of 80 sera from patients with ITP (37 acute, 43 chronic) 3 had positive results for anti TPO antibody. Specificity of these ELISA activities was analized by adsorption test, none of which revealed specific antibody activity. From these data we concluded that intrinsic anti TPO antibody is not likely to account for impaired megakaryocytopoiesis in ITP patients.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] 藤沢 康司: "特発性血小板減少性紫斑病の基礎と臨床"日本小児血液学会会誌. 16. 109-122 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 藤沢 康司: "State of the Art in Immunology, Hematology and Infection ITPの病態と治療-細菌の知見-"共和企画, 東京. 20 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Fujisawa K: "Pathophysiology and management of childhood idiopathic thrombocytopenic purpura."Jap J Pediatr Hematol. 16. 109-122 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Fujisawa K: "Recent advance in management of idiopathic thrombocytopenic purpura"State of the Art in Immunology, Hematology and Infection. (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 藤沢 康司: "特発性血小板減少性紫斑病の基礎と臨床"日本小児血液学会会誌. 16. 109-122 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 藤沢 康司: "State of the Art in Immunology, Hematology and Infection ITPの病態と治療-最近の知見-"共和企画,東京. 20 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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