DNA methylation of tumor suppressor gene in the development and in the metastasis of skin cancers
| Project/Area Number |
13670870
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | NIIGATA UNIVERSITY |
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Principal Investigator |
FUJIWARA Hiroshi NIIGATA UNIVERSITY Graduate School, Medical and Dental Sciences, Lecturer, 大学院・医歯学総合研究科, 講師 (20238637)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2001: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | p16 / p14 / E-cadherin / malignant melanoma / squamous cell carcinoma / basal cell carcinoma / methylation / cytosine extension assay / DNA / ケラチノサイト / メラノサイト / 末梢血リンパ球 |
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| Research Abstract |
Epigenetic regulation of the expression of tumor suppressor genes plays important role in tumor development. DNA methylation of gene promoter is a major pathway of the epigenetic regulation, however, its detail has not well studied in skin neoplasms.
With the power of non-isotopic cytosine extension assay, which we established recently, we demonstrated that malignant melanoma showed CpG-island specific hypermethylation compared with benign melanocytic nevus. Also, squamous cell carcinoma and basal cell carcinoma revealed CpG-island-specific hypomethylation.
Methylation-specific PCR revealed p16 promoter methylation in 70% of SCC, and 30% of BCC or melanoma. p14 promoter methylation was observed in small number of SCC. E-cadherin promoter methylation was most ubiquitously observed; in all the cases of SCC, halves the cases of BCC and melanoma.
Promoter DNA methylation may play an important role in the development of skin neoplasms.
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Report
(3 results)
Research Products
(3 results)
