In vivo role of adhesion molecules in inflammation induced by immur complex deposition
| Project/Area Number |
13670873
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Kanazawa University |
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Principal Investigator |
SATO Shinichi Kanazawa Univ. Grad. Sch. Of Medical Science Dermatology Associate Professor, 大学院・医学系研究科, 助教授 (20215792)
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| Co-Investigator(Kenkyū-buntansha) |
TAKEHARA Kazuhiko Kanazawa Univ. Grad. Sch. Of Medical Science Dermatology Professor, 大学院・医学系研究科, 教授 (50142253)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2002: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2001: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | Immune complex / L-selectin / ICAM-1 / Cell adhesion molecule / vasculitis / Arthus reaction / L-selectin |
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| Research Abstract |
The deposition of immune complexes (IC) induces an acute inflammatory response with tiss* injury. IC-induced inflammation is mediated by inflammatory cell infiltration, a process high* regulated by expression of multiple adhesion molecules. To assess the role of L-selectin ar* intercellular adhesion molecule-1 (ICAM-1) in this pathogenetic process, the cutaneous rever* passive Arthus reaction was examined in mice lacking L-selectin (L-selectin-/-), ICAM-1 (ICAM-1-/* or both (L-selectin/ICAM-1-/-). Edema and hemorrhage, which peaked 4 and 8 hours after * challenge respectively, were significantly reduced in L-selectin-/-, ICAM-1-/-, and L-selectin/ICAM* 1-/- mice compared with wild type littermates. In general, edema and hemorrhage were mo significantly inhibited in ICAM-1-/- mice than in L-selectin-A mice, but were most significant* reduced in L-selectin/ICAM-1-/- mice compared with ICAM-1-/- or L-selectin-/- mice. Decrease edema and hemorrhage correlated with reduced neutrophil and mast cell infiltration in all adhesi* molecule-deficient mice, but leukocyte infiltration was most affected in L-selectin/ICAM-1-/- mic Reduced neutrophil and mast cell infiltration was also observed for all mutant mice in the peritone Arthus reaction. Furthermore, cutaneous tumor necrosis factor-a production was inhibited in ea* deficient mouse, which paralleled the reductions in cutaneous inflammation. These resu* indicate that ICAM-1 and L-selectin cooperatively contribute to the cutaneous Arthus reaction * regulating neutrophii and mast cell recruitment and suggest that ICAM-1 and L-selectin a therapeutic targets for human IC-mediated disease.
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Report
(3 results)
Research Products
(3 results)
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[Publications] Kaburagi Y, Hasegawa M, Nagaoka T, Shimada Y, Hamaguchi Y, Komura K, Saito E, Yanaba, K, Takehara K, Kadono T, Steeber DA, Tedder TF, Sato S.: "The cutaneous reverse Arthus reaction requires intercellular adhesion molecule-1 and L-selectin expression"J Immunol.. 168. 2970-2978 (2002)
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