| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2002: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2001: ¥900,000 (Direct Cost: ¥900,000)
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| Research Abstract |
In this study, we investigated the suppressive effects of nutritional modification on allergic dermatitis, and also investigated the role of oxidative stress on inflammation in allergic dermatitis during nutritional modification.
NC/Nga mice, which spontaneously develop atopic dermatitis, were divided into ad libitum feeding group and diet restriction group given 60% of amount of ad libitum feeding group. They were observed for 9 weeks. The onset and progression of spontaneous dermatitis was delayed in the diet restriction group.
ICR mice were divided into ad libitum feeding group, 24-hour fasting group, and 48-hour fasting group. All mice were elucidated allergic contact dermatitis by painting of 2, 4-dinitrofluorobenzene on the ear. In the fasted mice, inflammation in ear was significantly suppressed than that in ad libitum feeding mice. These results were also confirmed semi-quantitatively by computed image analysis. Further, The level of oxidative stress was evaluated by immunohistochemistry using the monoclonal antibody to 8-hydroxydeoxyguanosine (8-OHdG), a biomarker for oxidative damage to DNA. The number of 8-OHdG stained nuclear in the fasted mice was also significantly less than that in ad libitum feeding mice.
In conclusion, moderate dietary restriction or short-term fasting showed the suppressive effects of allergic dermatitis, and the level of oxidative stress to DNA was suppressed according to suppression of dermatitis.
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