• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Study of Post-UV DNA Repair Factors for the Prevention from Photodamage of the Skin

Research Project

Project/Area Number 13670877
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Dermatology
Research InstitutionHamamatsu University School of Medicine

Principal Investigator

MORWAKI Shinichi  Hamamatsu University School of Medicine, Photon Medical Research Center, Associate Professor, 光量子医学研究センター, 助教授 (40303565)

Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2003: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2001: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsUltraviolet / DNA repair / Aging / Skin cancer / DNA polymerase δ / fibroblast / Nucleotide Excision Repair / ヌクレオチド除去修復 / 皮膚老化 / DNA損傷
Research Abstract

A reduction in the post-ultraviolet (UV) DNA repair capacity is associated with aging. To clarify the mechanism of this change, we examined the DNA repair capacity of skin fibroblasts from healthy donors of different ages by the two methods: host cell reactivation (HCR) assay and enzyme-linked immunosorbent assay (ELISA) of cyclobutane pyrimidine dimers and pyrimidine-pyrimidone (6-4) photoproducts. In HCR assay, cells from elderly donors exhibited significant declines in the ability to restore transfected reporter DNA damaged by UV light. In contrast, the ability to remove DNA damage declined little with age in ELISA. These results imply that the age-sensitive step took place after the damage excision in nucleotide excision repair (NER). The mRNA expression of DNA repair synthesis-related genes (DNA polymerase d, replication factor C, and proliferating cell nuclear antigen) were markedly decreased in the cells from multiple elderly subjects compared with those from young subjects. Further, the protein level of DNA polymerase d 1, a catalytic subunit of the pivotal factor in repair synthesis, correlated with the mRNA level. These findings suggest that the reduced post-UV DNA repair capacity in aging results from an impairment in the latter step of NER by the decreased expression of factors in repair synthesis.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (31 results)

All 2005 2004 2003 2002 2001 Other

All Journal Article (13 results) Book (1 results) Publications (17 results)

  • [Journal Article] Two New XPD Patients Compound Heterozygous for the same …2005

    • Author(s)
      Fujimoto M, et al.
    • Journal Title

      J Invest Dermatol (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Decreased Gene Expression Responsible for Post-Ultraviolet DNA …2005

    • Author(s)
      Takahashi K et al.
    • Journal Title

      J Invest Dermatol 124

      Pages: 435-442

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Decreased Gene Expression Responsible for Post-Ultraviolet DNA Repair Synthesis in Aging : A Possible Mechanism of Age-Related Reduction in DNA Repair Capacity.2005

    • Author(s)
      Takahashi Y., Moriwaki S., Sugiyama Y., Endo Y., Yamazaki K., Mori T., Takigawa M., Inoue S.
    • Journal Title

      J Invest Dermatol 124

      Pages: 435-442

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] 色素性乾皮症の現在2004

    • Author(s)
      森脇真一
    • Journal Title

      Visual Dermatology 3

      Pages: 182-185

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] 色素性乾皮症の診断と治療2004

    • Author(s)
      森脇真一
    • Journal Title

      Visual Dermatology 3

      Pages: 174-180

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Compound heterozygosity for the Xeroderma Pigmentosum …2002

    • Author(s)
      Negishi I et al.
    • Journal Title

      Eur J Dermatol 12

      Pages: 536-539

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] UV Dose Measurements of Photosensitive Dermatosis patients by …2002

    • Author(s)
      Yagi S et al.
    • Journal Title

      Photochemistry and Photobiology 76

      Pages: 669-671

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Augmentation of monocyte interleukin-8 production by psoralen/UVA-treated CD4+ T cells.2002

    • Author(s)
      Tokura Y., Takigawa M., Moriwaki S
    • Journal Title

      Exp Dermatol 11

      Pages: 564-572

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] UV dose measurements of photosensitive dermatosis patients by polycrystalline GaN-based portable self-data-acquisition UV monitor.2002

    • Author(s)
      Yagi S., Iwanaga T., Kojima H., Shoji Y., Suzuki S., Seno K., Mori H.,, Tokura Y., Takigawa M.Moriwaki M.
    • Journal Title

      Photochemistry and Photobiology 76

      Pages: 669-671

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Compound heterozygosity for the xeroderma pigmentosum complementation group A gene associated with a mild phenotype.2002

    • Author(s)
      Negishi I., Kato G., Moriwaki S., Ishikawa O.
    • Journal Title

      Eur J Dermatol 12

      Pages: 536-539

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Xeroderma pigmentosum【triple bond】bridging a gap between laboratory and clinic.2001

    • Author(s)
      Moriwaki S., Kraemer KH.
    • Journal Title

      Photoderm Photoimmun Photomed 17

      Pages: 47-54

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] 6p monosomy (6p25.1-pter) and 10q trisomy (10g25.2-qter) in a patient with DNA repair disturbance and premature senile syndrome.2001

    • Author(s)
      Akahoshi K., Moriwaki S., Fukushima Y., Takahashi I., Kimiya S.
    • Journal Title

      Am J Med Geneticss 101

      Pages: 153-157

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Lehmann AR Two new XPD patients compound heterozygous for the same mutation demonstrate diverse clinical features.

    • Author(s)
      Fujimoto M., Leech SN., Theron T., Fawcett H., Botta E., Mori M., Momoi M., Moriwaki S., Sfefanini M., Nakagawa H., Shuster S., Moss C.
    • Journal Title

      I Invest Dermatol (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Book] 皮膚疾患と看護B系統看護学講座専門22小児看護学2小児臨床看護各論2003

    • Author(s)
      森脇真一
    • Total Pages
      556
    • Publisher
      医学書院
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 森脇 真一: "色素性乾皮症の現在"Visual Dermatology. 3. 174-180 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 森脇 真一: "色素性乾皮症の診断と症例"Visual Dermatology. 3. 182-185 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 森脇 真一: "色素性乾皮症"皮膚疾患最新の治療. 77 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 森脇 真一: "色素性乾皮症 今日の小児治療指針"医学書院. 1 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Negishi I, Kato G, Moriwaki S, Ishikawa O: "Compound heterozygosity for the xeroderma pigmentosum complementation group A gene associated with a mild phenotype"Eur J Dermatol.. 12. 536-539 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tokura Y, Seo N, Tomida M, Sarukawa M, Hashizume H, Takigawa M, Moriwaki S: "Augmentation of monocyte interleukin-8 production by psoralen/UVA-treated CD4+ T cells"Exp Dermatol. 11. 564-572 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yagi S, Iwanaga T, Kojima H, Shoji Y, suzuki S, Seno K, Mori H, Tokura Y, Takigawa M, Moriwaki S: "UV dose measurements of photosensitive dermatosis patients by polycrystalline GaN-based portable self-data-aqcuisition UV monitor"Photochemistry and Photobiology. 76(6). 669-671 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 森脇真一: "色素性乾皮症"皮膚疾患最新の治療2003-2004(南江堂). 77 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 森脇真一: "光線過敏症 放射線による皮膚障害"エキスパートナーシング. 131-135,136-138 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 森脇真一: "色素性乾皮症"今日の小児治療指針(医学書院). (印刷中).

    • Related Report
      2002 Annual Research Report
  • [Publications] Moriwaki SI, Kraemer KH: "Xeroderma pigmentosum---bridging a gap between laboratory and clinic"Photoderm. Photoimmun. and Photomed.. 17. 47-54 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Grossmann L, Moriwaki SI et al.: "Age-associated changes in DNA repair and mutagenesis"The role of DNA Damage and Repair in Cell Aging (Elsevier). 17-30 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Akahoshi K, Moriwaki SI et al.: "6p monosomy (6p25.1-pter) and 10q trisomy (10q25.2-qter) in a patient with DNA repair disturbance and premature senile syndrome"Am. J. Med. Genetcs. 101. 153-157 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 森脇真一: "日焼けサロンと皮膚障害"こどもと健康. 67. 2-7 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 森脇真一: "光線過敏症"エキスパートナーシング. (印刷中).

    • Related Report
      2001 Annual Research Report
  • [Publications] 森脇真一: "色素性乾皮症"今日の小児治療指針 (医学書院). (印刷中).

    • Related Report
      2001 Annual Research Report
  • [Publications] 森脇真一: "色素性乾皮症"皮膚疾患再診の治療 2003-2004 (南江堂). (印刷中).

    • Related Report
      2001 Annual Research Report

URL: 

Published: 2001-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi