| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2001: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Research Abstract |
ADAM family is transtmembrane protein that shares a disintegrin domain and metalloproteinase domain. To date, more than 30 members have been identified, although their role and regulation in normal human keratinocytes have not been fully determined. In order to assess whether ADAM family play an important role for keratinocyte proliferation and differentiation, we investigated their expression in normal human keratinocytes. We constructed Taqman probe for 19 ADAM family members. Total RNA was harvested from normal human keratinocytes under serum free condition, and then the expression level was determined by real time polymerase chain reaction. Keratinocyte express 15 ADAM family members at proliferating phase, and ADAM9 was upregulated by the addition of EGF. To further characterize the involvement of ADAM in skin disease, we investigated ADAM expression in lesional epidermis of psoriasis vulgaris, a hyperproliferative disorder. Epidermis was separated by heat activation from 13 skin samples from psoriasis vulgaris and 11 skin samples from normal healthy skin, and total RNA was extracted. ADAM expression was determined by real time PCR. There is no difference about ADAM12, 21, TS3, TS4, TS5 and TS6 expression between psoriasis vulgaris and normal health skin, whereas ADAM10, 17, 20 and 28 were downregulated in psoriatic epidermis, and ADAM30 was upregulated in psoriatic epidermis. ADAM15 was dramatically downregulated in psoriatic epidermis. Furthermore, ADAM19 and ADAMTS7 were significantly upregulated in psoriatic epidermis. Taking together, we demonstrated that normal human keratinocytes express more than 15 ADAM families, and ADAM family might be involved in the pathogenesis of psoriasis vulgaris.
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