| Project/Area Number |
13670893
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Oita Medical University |
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Principal Investigator |
MATSUO Noritaka (2002) Oita Medical University, Medical Department, Research Assistant, 医学部, 助手 (10284788)
園田 忠重 (2001) 大分医科大学, 医学部, 助手 (80244169)
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| Co-Investigator(Kenkyū-buntansha) |
SONODA Yoshiko Oita Medical University, Medical Department, Research Assistant, 医学部, 助手 (80253780)
YOSHIOKA Hidekatsu Oita Medical University, Medical Department, Professor, 医学部, 教授 (00222430)
佐藤 俊宏 大分医科大学, 医学部, 助手 (70244176)
松尾 哲孝 大分医科大学, 医学部, 助手 (10284788)
浅田 裕司 大分医科大学, 医学部, 助手 (30284798)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2001: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | protease nexin-1 / SERPIN / hair follicle / hair cycle / protein expression / monoclonal antibody / polyclonal antibody |
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| Research Abstract |
1) Production of anti-protease nexin-1 (PN1) peptide antibody
Fourteen mer of peptides, which is corresponding to one of the predicted peptide regions with high antigenicity of rat PN1 amino acids, were synthesized. Two rabbits were immunized with the peptides conjugated with KLH as a carrier protein, and the antiserum was prepared.
2) Specificity of the anti-PN1 antibody
No reaction was detected with Western blot analysis between the anti-PN1 antibody and GST-PN1 fusion protein or native PN1 protein, which was purified from the conditioned medium of the cultured dermal papilla cells. No reaction was detected even when using the lgG purified from the antiserum.
3) Localization of PN1 protein in hair follicles
No reaction was detected with immunohistochemical examination with the anti-PN1 antibody in order to localize PN1 protein in hair follicles of human and mouse. Anti-PN1 polyclonal antibody, for which rat PN1 fusion protein expressed by pET system, was kindly gifted from a laboratory. But no reaction was detected with the same immunohistochemical examination.
These results suggested that an amount of PN1 protein in a hair follicle was much less than estimated.
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