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Cellular Analysis of Cutaneous Granuloma induced by Antigen-binding Agarose Particle and MDP

Research Project

Project/Area Number 13670903
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Dermatology
Research InstitutionKansai Medical University

Principal Investigator

OKAMOTO Hiroyuki  Kansai Med. Univ., Dermatology, Associate Professor, 医学部, 助教授 (10142291)

Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2001: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsGranuloma / Mice / MDP / Adjuvant / DNP / Monocyte / Giant Cell / T cell / MDP / 骨髄細胞 / インターフェロン / IL-3
Research Abstract

We examined immune cells and cytokine production in murine cutaneous granulomas induced by antigen-binding agarose particles and MDP. A few number of CD3+ cells are present in the center of granulomas and their surrounding areas. Almost same numbers of CD4+ and CD8+ cells are distributed. On the other hand, CD14+ monocytes are main cells of the granuloma, but tissure macrophages which are positive for MMGL were not present in the center of the granulomas. IFN-γ+ cells were more predominantly distributed than IL-4+ cells, suggesting that the induced cutaneous granuloma had a property of TH1 type reaction. To examine the propety of precursor cells of monocyte-macrophage lineage cells in the granulomas, the folowing cells were prepared from GFP-transgeneic mice, 1) peripheral monocytes, 2) macrophages induced by culturing monoctes, 3) MMGL+ dermal macrophages in normal skin, 4) peritoneal macrophages, 5) bone marrow cells, 6) dendritic cells induced from bone marrow cells with GM-CSF/IL-4. These cells were administered subcutaneously or intraveneously into granuloma-bearing mice. GFP+ cells were found in the granuloma only when freshly isolated bone marrow cells were injected. To investigate the response ofepithelioid cells in granuloma to cytokines, epithelioid cells were riched by eliminating CD3+ cells, B220+ cells and MMGL+ cells in the granulomas. Theses cells responded to IFN-γ and IL-3 but not to IL-13 or IL-10. These results suggest that infiltrating cells from bonw marrows consist of cutaneous granulomas induced by antigen-binding agarose particles and MDP, and that IFN-γ and EL-3 are a key factor for the formation of granulomas.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Hiroyuki Okamoto: "Laughans-type and foreign body-type multi-nucleated giant cells"Acta Dermatoveneol. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kana Mizuno: "Tuhi**ory influence of xanth** oxid**e inhibitor and Ale inhibitor in metiness**nes giou**cel formation from mono**o by downregulation of adhesion molecules and puri***ric receptors"Brit J Dermatol. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hiroyuki Okamoto: "Monocyte-derived multinucleates giant cells and sarcoillers"J.Dermatol Sci. 31. 119-128 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hiroyuki Okamoto: "Circulating CD14^+ CD16^+ monocytes are expanded in sarcoidosis patients"J.Dermatol. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hiroyuki Okamoto, Kana Mizuno, Takeshi Horio: "Monocyte-derived multinucleated giant cells and sarcoidosis."J Dermatol Sci. 31. 119-128 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hiroyuki Okamoto, Kana Mizuno, Takeshi Horio: "Langhans-type and foreign body-type multinucleated giant cells in cutaneous lesions of Sarcoidosis"Acta Dermatoveneol. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kana Mizuno, Hiroyuki Okamoto, Takeshi Horio: "Inhibitory influences of xanthine oxidase inhibitor and ACE inhibitor on multinucleated giant cell formation from monocytes by downregulation of adhesion molecules and purinergic receptors."Brit J Dermatol. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hiroyuki Okamoto, Kana Mizuno, Takeshi Horio: "Circulating CD14+CD16+ monocytes are expanded in sarcoidosis patients."J Dermatol. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Erina Okumura: "A case of cutaneous-type adult T-cell leukaemia/lymphoma showing granulome formation under a parapsoriatic eruption"Brit J Dermatol. 147. 1020-1022 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hiroyuki Okamoto: "Inhibition of Langerhans cell function by UVB radiation"J. Photoscience. 9. 190-193 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Naoko Uetsu: "Treatment of chronic actinic dermatitis with tacrolimus ointment"J. Amer. Acad. Dermatol. 47. 881-884 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hiroyuki Okamoto: "Langhans-type and foreign body-type multi nucleated giant cells"Acta Dermatoveneol. (印刷中).

    • Related Report
      2002 Annual Research Report
  • [Publications] Kana Mizuno: "Inhibitory influences of xanthine oxidase inhibitor and ACE inhibitor on multinucleated giant cell formation from monogtes by down regulation of adhesion molecules and purinergic receptors"Brit J Dermatol. (印刷中).

    • Related Report
      2002 Annual Research Report
  • [Publications] Hiroyuki Okamoto: "Monocyte-derived multinucleated giant cells and sarco-closis"J. Dermatol Sci. (印刷中).

    • Related Report
      2002 Annual Research Report
  • [Publications] Kana Mizuno: "Predominaut Induction of Langhans tepe multineecleated Giaut Cells in uitrcfucn Human Monccytes by Muramyl Dipeplted and the Supernatout of Coucanaralin. A-stimulated mononuclear ceas"Journal of Leulcocyte Biology. 70. 386-394 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kana Mizuno: "Heightened ability of monocytes from sarco : dosis patients ts form multi-neecleated gicmt cells in vitor by supernatouts of concanavalin A-stimulates mono necclew cell"Clinical Experimental Immurology. 126. 151-157 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kaoru Mitsuya: "Comprehensive analysis of 28 patieits with latey alleigy and prevalence of latex 3eusitization among hospital persornel"Journal of Derwatology. 405-412 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takeshi Horio: "Phetobiologic and photoimmunologic characteristics of XPA gene-deficient mice"Journal of Invoshigahve Dermotological Symposium Proc.. 6. 58-63 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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