| Project/Area Number |
13670919
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | Chiba University |
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Principal Investigator |
ITO Hisao Chiba University, Graduate School of Medicine, Professor, 大学院・医学研究院, 教授 (20095574)
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| Co-Investigator(Kenkyū-buntansha) |
ARUGA Takashi Chiba University, University Hospital, Assistant, 医学部附属病院, 助手 (20261901)
KAWATA Tetusya Chiba University, Graduate School of Medicine, Lecturer, 大学院・医学研究院, 講師 (60234077)
UNO Takashi Chiba University, Graduate School of Medicine, Lecturer, 大学院・医学研究院, 講師 (30302540)
ISOBE Koichi Chiba University, University Hospital, Assistant, 医学部附属病院, 助手 (80334184)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2002: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2001: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | FISH / Chromosomal aberration / Radiosensitivity / Radiotherapy / 染色体変 / AT細胞 / DNA損傷修復 |
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| Research Abstract |
There are many studies to analyze the chromosomal aberration after irradiation to the cancer cells and normal lymphocytes. In the conventional chromosome analysis, the cells in the M-phase can be the target. However, the efficiency of this method was very low and sufficient results could not be expected. When the transformation of the chromosome was adapted as the index, the sensitivity of this method was also low. Also, it was impossible to detect the chromosomal translocation. As a result, analysis of chromosome aberration had been a tough business. The condensed chromosomes with Calicrein A were stained with fluorescent dyes for the specific chromosome in this study instead of the conventional method. In this study, we planned to analyze the chromosomal aberrations in the irradiated cells and discover the relationship between radiosensitivity and abnormal chromosomal aberrations. In 2001, radiosensitive fibroblast, AT cells, and normal fibroblast cells were irradiated. It was found that AT cells have poor repair of DNA damage, especially G1 stage, after irradiation, comparing to normal fibroblasts. In 2002, transformation of chromosomes and translocation after irradiation were analyzed with FISH in irradiated AT cells and normal fibroblasts. As a results, it was determined that radiosensitive fibroblasts made more missrepairing. When heavy ions (carbon beams) was irradiated to fibroblasts, there appeared much more fragments than expected from radiosensitivity. We could not get the definitive explanation about the radiosensitivity and chromosomal aberration. This study will be continued with tumor cells in the future.
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