• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of receptor activation method for brain imaging with PET or SPECT

Research Project

Project/Area Number 13670935
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Radiation science
Research InstitutionOsaka University

Principal Investigator

INOUE Osamu  Faculty of Medicine, Professor, 医学部, 教授 (50159969)

Co-Investigator(Kenkyū-buntansha) SUZUKI Kazutoshi  National Institute of radiological Sciences Head, Radiopharmaceutical Chemistry Section, ユニット長(研究職) (90162932)
HOSOI Rio  Faculty of Medicine, Staff, 医学部, 教務職員 (30291446)
KOBAYASHI Kaoru  Faculty of Medicine, Associate Professor, 医学部, 助教授 (90256933)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2001: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordscAMP / NO / receptor binding / in vivo / in vitro / インビドロ
Research Abstract

Effects of rolipram, a selective phosphodiesterase type IV inhibitor, upon dopamine D_1 and dopamine D_2 receptor biding in mouse brain were investigated. Rolipram significantly and dose-dependently decreased both dopamine D_1 and dopamine D_2 receptor binding in mouse striatum. The kinetic analysis of ^3H-SCH23390 binding revealed that rolipram decreased the input rate constant (k_3=Kon/Bmax) of ^3H-23390 binding. In vivo saturation experiment on ^3H-SGH23390 binding showed no significant change in Bmax of D_1 receptor in rolipram-treared mice. A significant reduction in in vivo binding of muscarinic acetyloholine receptor was also observed by pretreated with rolipram. As rolipram increased cAMP content in the brain, these results indicated an important role of cAMP upon receptor biding in intact brain.
Effects of microinjection of db-cAMP into rat striatum on ^3H-SGH23390 binding were also examined by autoradiography. Db-cAMP significantly and dose-dependently increased ^3H-SCH23390 b … More iding in rat striatum. This increase in binding was almost completely inhibited by pretreated with Rp-cAMPs, a protein kinase A(PKA) inhibitor, which indicated an important role of cAMP/PKA system on dopamine D_1 receptor biding. ^3H-NMSP biding as well as ^3H-NMPB binding was also increased by microinjection of db-cAMP, which indicated an important role of cAMP/PKA system on dopamine D_1 receptor biding. ^3H-NMSP biding as well as ^3H-NMPB biding was also increased by microinjection of db-cAMP, which indicated global changes in microenvironment induced by phospholylation of protein might be occurred.
Another important finding is that Rp-cAMPs significantly increased glucose metabolism in rat brain although the regional blood flow was decreased. The increase in glucose metabolism was found to be due to increase in phospholylation process of glucose by hexokinase.
In conclusion, cAMP/PKA system has important role on molecule interaction process including ligand-resepter interaction and substrate-enzyme reaction in intact brain. Less

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Hosoi-R et al.: "Effect of rolipram muscarinic acethylcholine receptor binding in the intact mouse brain"J-Neural Transmission. 110. 363-372 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hosoi-R et al.: "Effect of rolipram on vivo dopamine receptor binding"J-Neural Transmission. 109. 1139-1149 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Abe-K et al.: "Increment of in vivo binding of [3H]-SCH23390, a dopamine D1 receptor ligand, induced by cyclic AMP-dependent protein kinase in rat brain"Brain-Res. 952. 211-217 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ishikawa-M et al.: "Rolipram depresses [3H]-2-deoxyglucose uptake in mouse brain and heart in vivo"Eur-J-Nucl-Med. 29. 1212-1215 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kobayashi-K et al.: "Enhancement of the relative uptake of 18F-FDG in mouse fibrosarcoma by rolipram"Ann-Nucl-Med. 16. 507-510 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hosoi-R et al.: "Effect of rolipram on musoarinic acetylcoline Receptor biding in the intact mouse brain."J Neural Transm. 110(4). 363-372 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hosoi-R et al.: "Effects of rolipram on dopamine receptor binding."J Neural Transm. 109. 1139-1149 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Abe-K et al.: "Increment of in vivo binding of [^3H]-SCH23390, a dopamine D_1 receptor ligand, induces by cyclic AMP-dependent protein kinase in rat brain."Brain-Res. 952(2). 211-217 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ishikawa-M et al.: "Rolipram depresses [^3H]-2-deoxyglucose uptake in mouse brain and heart vivo."Eur J Nucl Med. 29. 1212-1215 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kobayashi-K et al.: "Enhancement of the relative uptake of ^<18>F-FDG in mouse fibrosarcome by rolipram."Ann Nucl Med. Nov:16(7). 507-510 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hosoi-R et al.: "The role of the cAMP-PKA system in the short-term regulation of striatal [^<11>C]-2-deoxyglucose uptake in freely moving rats."Brain-Res. 921. 260-263 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hosoi-R et al.: "Effect of rolipram on in vivo dopamine receptor binding"J-Neural Transmission. 109. 1139-1149 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Abe-K et al.: "Increment of in vivo binding of [3H]-SCH23390, a dopamine D1 receptor ligand, induced by cyclic AMP-dependent protein kinase in rat brain"Brain-Res.. 18;952(2). 211-217 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ishikawa-M et al.: "Rolipram depresses [3H]-2-deoxyglucose uptake in mouse brain and heart in vivo"Eur-J-Nucl-Med. 29. 1212-1215 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kobayasshi-K et al.: "Enhancement of the relative uptake of 18F-FDG in mouse fibrosarcoma by rolipram"Ann-Nucl-Med. 16(7). 507-510 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hosoi R et al.: "The role of the cAMP-PKA system in the short-term regulation of striatal [14C]-2-deoxyglucose uptake in freely moving rats"Brain Res.. 921. 260-263 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hosoi-R et al.: "The role of cAMP-PKA system in the short-term regulation of striatal[14C]-2-deoxyglucose uptake in freely moving rats"Brain Research. 921. 260-263 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hosoi-R et al.: "Effect of roliprem on in vivo dopamine receptor binding"J-Neural Transmission. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Fujino-K et al.: "Effect of acute physiological stressors on serotonin release in mice brain monitored by microdialysis"Neuroscience letters. (in press).

    • Related Report
      2001 Annual Research Report

URL: 

Published: 2001-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi