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Mechanism of radiation-induced apoptosis of human peripheral T cells

Research Project

Project/Area Number 13670948
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Radiation science
Research InstitutionKochi Medical School

Principal Investigator

OGAWA Yasuhiro  Kochi Medical School, Faculty of Medicine, Associate Professor, 医学部, 助教授 (90152397)

Co-Investigator(Kenkyū-buntansha) NISHIOKA Akihito  Kochi Medical School Faculty of Medicine, Instructor, 医学部, 助手 (70237668)
KOBAYASHI Toshihiro  Kochi Medical School Faculty of Medicine, Instructor, 医学部, 助手 (40153621)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2001: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsLymphocyte / T cell / Apoptosis / Radiation / Mitochondria
Research Abstract

The mechanism leading to the high level of radiosensitivity of T lymphocytes has not yet been fully described. In our previous study, we demonstrated that human peripheral T lymphocytes revealed early apoptotic changes (annexin-V-positive) and late apoptotic changes (propidium iodide-positive), at 13 and 24 h after irradiation of 5 Gy, respectively. Changes in mitochondrial membrane potential were observed at 10 h after irradiation of 5 Gy. Mitochondrial cytochrome c release was confirmed following the changes in mitochondrial membrane potential. In order to elucidate the mechanism which occurs prior to the mitochondrial membrane potential changes, we examined the radiation dose and the timing of oxidative DNA damage induced in human peripheral T lymphocytes following 10 MV X-ray irradiation. As a result, the production of 8-oxoguanine, i. e., the product of oxidative DNA damage, was clearly identified starting at 10, 6, and 3 h, after 2, 5, and 20 Gy of irradiation, respectively.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] 戸田巌雄: "The role of apoptotic resistance in irradiated adult articular chondrocytes"Int. J. Mol. Med.. 9. 349-352 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 小川恭弘: "Mitochondrial cytochrome c release in radiation-induced apoptosis of human peripheral T cells"Int. J. Mol. Med.. 10. 263-268 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 小川恭弘: "Radiation-induced oxidative DNA damage, 8-oxoguanine, in human peripheral T cells"Int. J. Mol. Med.. 11. 27-32 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 小川恭弘: "Radiation-induced reactive oxygen species formation prior to oxidative DNA damage in human peripheral T cells"Int. J. Mol. Med.. 11. 149-152 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 高橋敏明: "Role of apoptosis inhibition in various chondrocyte culture systems"Int. J. Mol. Med.. 11. 29-303 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Toda I, et al.: "The role of apoptotic resistance in irradiated adult articular chondrocytes"Int J Mol Med.. 9. 349-352 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ogawa Y, et al.: "Mitochondrial cytochrome c release in radiation-induced apoptosis of human peripheral T cells"Int J Mol Med.. 10. 263-268 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ogawa Y, et al.: "Radiation-induced oxidative DNA damage, 8-oxoguanine, in human peripheral T cells"Int J Mol Med.. 11. 27-32 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ogawa Y, et al.: "Radiationm-induced reactive oxygen species formation prior to oxidative DNA damage in human peripheral T cells"Int J Mol Med.. 11. 149-152 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takahashi T, et al.: "Role of apoptosis inhibition in various chondrocyte culture systems"Int J Mol Med.. 11. 299-303 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 戸田巌雄: "The role of apoptotic resistance in irradiated adult articular chondrocytes"Int. J. Mol. Med.. 9. 349-352 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 小川恭弘: "Mitochondrial cytochrome c release in radiation-induced apoptosis of human peripheral T cells"Int. J. Mol. Med.. 10. 263-268 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 小川恭弘: "Radiation-induced oxidative DNA damage, 8-oxoguanine, in human peripheral T cells"Int. J. Mol. Med.. 11. 27-32 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 小川恭弘: "Radiation-induced reactive oxygen species formation prior to oxidative DNA damage in human peripheral T cells"Int. J. Mol. Med.. 11. 149-152 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 高橋敏明: "Role of apoptosis inhibition in various chondrocyte culture systems"Int. J. Mol. Med.. 11. 299-303 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 小川恭弘: "Radiation-induced apoptosis of human peripheral T cells : analyses with cDNA expression arrays and mitochondrial membrane potential assay"Int.J.Mol.Med.. 7. 603-607 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 小川恭弘: "Sudden termination of G-CSF injection leads to apoptosis of a large proportion of increased granulocytes"Oncology Reports. 8. 1027-1029 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 小林俊博: "スーパーオキサイドの細胞内検出法"細胞. 33. 272-275 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 武内直子: "Scintigraphic prediction of response to chemotherapy in patients with breast cancer : Technetium 99m-tetrofosmin and thallium-201 dual single photon emission computed tomography"Int.J.Oncol.. 20. 53-58 (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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