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A molecular pathogenetic study of anxiety disorders in the brain.

Research Project

Project/Area Number 13670989
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Psychiatric science
Research InstitutionTOKYO MEDICAL AND DENTAL UNIVERSITY

Principal Investigator

KURUMAJI Akeo  TOKYO MEDICAL & DENTAL UNIVERSITY, SCHOOL OF MEDICINE, LECTURER, 医学部附属病院, 講師 (00251504)

Co-Investigator(Kenkyū-buntansha) KASHIWA Atsushi  TOKYO MEDICAL & DENTAL UNIVERSITY, GRADUATE SCHOOL, ASSISTANT, 大学院・医歯学総合研究科, 助手 (10301227)
NISHIKAWA Toru  TOKYO MEDICAL & DENTAL UNIVERSITY, GRADUATE SCHOOL, PROFESSOR, 大学院・医歯学総合研究科, 教授 (00198441)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2002: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2001: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsANXIETY DISORDERS / NEURODEVELOPMENT / DNA MICROARRAY / PHARMACOLOGICAL STRESSOR / GABA / IMMEDIATE EARY GENE / CERFERAL CORTEX / RAP PCR / 大脳心皮質
Research Abstract

We examined effects of FG 7142 (20 mg/kg, I.p.), an axiogenic stressor, on c-Fos-like immunoreactivity in the rat brain. In the forebrain of 56-day-old rats, the drug induced a dense nuclear c-Fos immunostaining in the piriform cortex, cingulate and retosplenial cortex, layers II-IV of the neocortex, lateral habenula, lateral sepum, striatum, central and medial nucleus of the amygdala, but a sparse c-Fos immunostaining in the hippocampus and layer I of the neocortex. Among these regions, 8-day-old rats expressed much fewer c-Fos-positive cells in the cingulate and retosplenial cortex, neocortex, lateral habenular, lateral septum and medial nucleus amygdala than young adult rats after the FG 7142 administration. These difference in the regional distribution of a neural activity marker, c-Fos, could reflect the postnatal developmental of neuronal populations or neuron circuits involved in stress response and/or anxiety in the brain.
To determine the genes responsible for stress or anxiety state in the brain of rodents, transcriptional changes in the cerebral cortex after the FG 7142 injection were rnonitered using DNA microarray. In the young adult rodents, a number of genes, such as c-fos and CYR 61, were significantly changed in the transcriptional levels. However, no changes in the levels in the cortex were observed in any of genes examined in the array in 8-day- old rodents. Thus, the gene exhibited age-dependent changes in the transcriptional levels following FG 7142 could play an important role in the neuonal circuits of stress response and/or anxiety state in the brain.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Akeo Kurumaji et al.: "An association study between polymorphism of L1CAM gene and schizophrenia in a Japanese sample"Am J of Med Genet (Neuropsychiatry Genetics). 105. 99-104 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takeo Yoshikawa et al.: "Evidence for association of the myo-inositol monophosphatase 2 (IMPA2) gene with schizophrenia in Japanese samples"Molecular Psychiatry. 6. 202-210 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] A.Kurumaji, et al.: "An association of the polymorphic repeat of tetranucleotide (TCAT) in the first intron of human tyrosine hydroxylase gene with schizophrenia"Journal of Neural Transmission. 108. 489-495 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Akeo Kurumaji, et al.: "No association of two missense variations of the benzodiazepine receptor (peripheral) gene and mood disorders in a Japanese sanple"Am J of Med Genet (Neuropsychiatry Genetics). 105. 172-175 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Ishiguro, et al.: "Association analysis of the pituitary adenyl cyclase activating peptide gene (PACAP) on chromosome 18p11 with schizophrenia nenndo"Journal of Neural Transmission. 108. 849-854 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 車地 暁生, 西川 徹: "ストレス応答関連遺伝子と神経回路-ストレスによるc-fosとCRHの遺伝子発現の変化"医学のあゆみ. 197. 263-266 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 車地 暁生: "コメディカルの治療薬マニュアル"中外医学社. 399-424 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kurumaji A, Nomoto H, Okano T, Toru M.: "An association study between polymoiphism of LICAM gene and schizophrenia in a Japanese sample"Am J Med Genet 2001. 105. 99-104

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yoshikawa T, Kikuchi M, Saito K, Watanabe A, Yamada K, Shibuya H, et al: "Evidence for association of the myo-inositol monophosphatase 2 (IMPA2) gene with schizophrenia in Japanese samples"Mol Psychiatry 2001. 6. 202-210

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kurumaji A, Kuroda T, Yamada K, Yoshikawa T, Toru M: "An association of the polymorphic repeat of tetranucleotide (TCAT) in the first intron of the human tyrosine hydroxylase gene with schizophrenia in a Japanese sample"J Neural Transm 2001. 108. 489-495

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kurumaji A, Nomoto H, Yamada K, Yoshikawa T, Toru M: "No association of two missense variations of the benzodiazepine receptor (peripheral) gene and mood disorders in a Japanese sample"Am J Med Genet 2001. 105. 172-175

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ishiguro H, Ohtsuki T, Okubo Y, Kurumaji A, Arinami T: "Association analysis of the pituitary adenyl cyclase activating peptide gene (PACAP) on chromosome 18p11 with schizophrenia and bipolar disorders"J Neural Transm 2001. 108. 849-854

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 車地 暁生, 西川 徹: "ストレス関連遺伝子"分子精神医学. 2. 205-210 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 車地 暁生: "神経発達からみた統合失調症"精神科. 1. 299-304 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 車地 暁生: "コメディカルの治療薬マニュアル"中外医学社. 399-424 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Akeo Kurumaji, et al.: "An association study between polymorphism of L1CAM gene and schizophrenia in a Japanese sample"Am J of Med Genet(Neuropsychiatry Genetics). 105. 99-104 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takeo Yoshikawa, et al.: "Evidence for association of the myo-inositol monophosphatase 2(IMPA2) gene with schizophrenia in Japanese samples"Molecular Psychiatry. 6. 202-210 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] A.Kurumaji, et al.: "An association of the polymorphic repeat of tetranudeotide (TCAT) in the first intron of human tyrosine hydroxylase gene with schizophrenia…"Journal of Neural Transmission. 108. 489-495 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Akeo Kurumaji, et al.: "No association of two missense variations of the benzodiazepine receptor (peripheral) gene and mood disorders in a Japanese sanple"Am J Med Genet(Neuropsychiatry Genetics). 105. 172-175 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] H.Ishiguro, et al.: "Association analysis of the pituitary adenyl cyclase activating peptide gene (PACAP)on chromosome 18p11 with schizophrenia…………nenndo"Journal of Neural Transmission. 108. 849-854 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 車地暁生, 西川 徹: "ストレス応答関連遺伝子と神経回路-ストレスによるc-fosとCRHの遺伝子発現の変化"医学のあゆみ. 197. 263-266 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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