| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2001: ¥2,900,000 (Direct Cost: ¥2,900,000)
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| Research Abstract |
Some psychiatric disorders seem to have a basis in a dysfunction of the brain dopamine systems. Dopamine transporter (DAT) is a dopamine specific monoamine transporter that acts to take released dopamine back up into presynaptic terminals, so DAT gene expression seems to control the transmission of dopaminergic neurons. Since psyshotropic agents such as cocaine, amphetamine, phencyclidine and 1-methyl-4-phenylpyridinium (MPP) bind to DAT, DAT gene would be one of the targets for substance abuse. Our group fas reported that human dopamine transporter gene (DAT1) has a variable number of tandem repeats (VNTR) in its 3'-UTR of the DAT1 gene, 2319G/A, and a significant association between this polymorphism in the haplotype analysis with G2319A-and VNTR-polymorphisms. In this study, I found two novel polymorphisms in the 5'-flanking region of DAT gene, -1173C/G and -843C/T. since both polymorphisms seem to be functional because of the analisis with a computer transcription factor search program, I studied the association study between control subjects (n=106) and alcoholic subjects (n=182). Unfortunately, I could not find any significance between them concluded that further studies will be needed.
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