| Project/Area Number |
13671016
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Psychiatric science
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| Research Institution | Nagasaki University |
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Principal Investigator |
IMAMURA Akira Nagasaki University, Graduate School of Biomedical Sciences, Lecturer, 大学院・医歯薬学総合研究科, 講師 (40325642)
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| Co-Investigator(Kenkyū-buntansha) |
NAKANE Yoshibumi Nagasaki International University, Professor, 人間社会学部, 教授 (80039833)
HAYASHIDA Masaki Health Care Center, Associate Professor, 保健管理センター, 助教授 (70264223)
TSUJITA Takahiro Hironaka Hospital, Medical Doctor, 医師 (40304919)
OKAZAKI Yuji Mie University, Neuopsychiatry, Professor, 医学部, 教授 (40010318)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2001: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | schizophrenia / anticpation / monozygotic twin / DNA methylation / methylation-sensitive restriction enzyme / HPLC / 精神分裂病(統合失調症) / 遺伝子発現調整 / RLGS法 / M-PCR法 / 精神分裂病 |
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| Research Abstract |
The aim of this study was to explore the molecular mechanisms including epigenetic DNA modification underlying genetic anticipation in schizophrenia. Twelve two-generation pairs with schizophrenia, which showed prominent anticipation., were examined.
First, we investigated whether the promoter region of dopamine D2 receptor gene (DRD2) was methylated or not, using the methylation-sensitive restriction enzyme, Hap II, and the methylation-insensitive enzyme, MspI. After enzyme digestion, polymerase chain reaction (PCR) was performed, and. the. size of PCR products was determined with polyacrylamide gel electrophoresis. No difference was found between two generation in methylation of the promoter region of DRD2.
Second, we measured 5-methyl-2'-deoxycytidine (mC) contents by high performance liquid chromatography (HPLC) in peripheral leukocytes DNA obtained from eight two-generation pairs with schizophrenia, to compare the global methylation levels between two generation. We obtained no significant results in this method.
In summary, we found no evidence for the relationship between genetic anticipation and DNA methylation in schizophrenia. Therefore in future studies we will aim to collect more subjects and try other enzymes.
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