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Establishment of ?-secretes assay and functional analysis of Presenilin

Research Project

Project/Area Number 13671024
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Psychiatric science
Research InstitutionYOKOHAMA CITY UNIVERSITY

Principal Investigator

MIURA Satoshi  YOKOHAMA CITY UNIV., Medical, Associate Prof., 医学部, 助教授 (60157427)

Co-Investigator(Kenkyū-buntansha) NAKAI Toshiki  YOKOHAMA CITY UNIV., Medical, Assistant Prof., 医学部, 助手 (20270712)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2002: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2001: ¥1,900,000 (Direct Cost: ¥1,900,000)
Keywordspresenilin / ?-secretes
Research Abstract

Establishment of γ-secretes assay
In this study, we made several fusion proteins such as APPC100-HA, APPC100-Frag, APPC100-leader-peptidase, NotchC100-HA, NotchC100-Frag and NotchC100-leader-peptidase which were thought to be direct substrates for γ-secretes. Though we have not succeeded in detecting the γ-secretes activities, radiolabeled these polypeptides are most favorable substrates detecting the γ-secretes activities in quantative fashion. We are now optimizing the assay conditions in detail for ?-secretes activities.
Analysis of high molecular weight protein complex including presenilin.
Presenilin has been reported to form a large protein complex with many other membrane proteins which has an activity of γ-secretes. We analyzed solubilized rat liver micro some fraction with many kind of detergents by Blue-Native-Gel -Electrophoresis (BN-PAGE) and glycerol-density-gradient centrifugation, and found that digitonin solubilized presenilin complex is larger than 250kDa that is previously reported from another laboratories. We are now investigating the minimum components of presenilin complex having γ-secretes activity.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] W.Marui, E.Iseki, T.Nakai, S.Miura, M.Kato, K.Kosaka: "Progression and staging of Lewy pathology in brains from patients with dementia with Lewy bodies"J. Neurol. Sci.. 195. 153-159 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] E.Iseki, N.Takayama, Y.Furukawa, W.Marui, T.Nakai, S.Miura, K.Ueda, K.Kosaka: "Immunohistochemical study of synphilin-1 in brains of patients with dementia with Lewy bodies -synphilin-1 is non-specifically implicated in the formation of different nueronal cytoskeletal inclusions"Neurosci. Lett.. 326. 211-215 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] W. Marui, E Iseki, T. Nakai, S. Miura, M. Kato and K.kosaka: "Progression and staging of Lowy pathology in brains from patients with dementia with Lowy bodies."J. Neuro1. Sci.. 195. 153-159 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] E. Iseki, N. Takayama, Y. Furukawa, W. Marui, T. Nakai, S. Miura, K. Ueda and K. Kosaka: "Immunohistochemical study of synphilin-1 in brains of patients with dementia with Lowy bodies-synphilin-1 is non-specifically implicated in the formation of different neuronal cytoskeleton inclusions."Neurosci. Lett.. 326. 211-215 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary

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Published: 2001-04-01   Modified: 2016-04-21  

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