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Molecular biological approach to investigate higher brain functions

Research Project

Project/Area Number 13671027
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Psychiatric science
Research InstitutionTokyo Metropolitan University of Health Sciences (2002)
Jichi Medical University (2001)

Principal Investigator

SUDA Haruhiko (2002)  Tokyo Metropolitan University of Health Sciences Professor, 保健科学部, 教授 (40051784)

丹生谷 正史 (2001)  自治医科大学, 医学部, 助手 (00228256)

Co-Investigator(Kenkyū-buntansha) NISHIJIMA Koichi  Jichi Medical School Associate Professor, 精神医学, 助教授 (30198460)
NIBUYA Masashi  National Defense Medical College Lecturer, 精神科学, 講師 (00228256)
TATEYAMA Masato  Tokyo Metropolitan University of Health Sciences Professor, 保健科学部, 教授 (60118966)
鈴木 映二  北里大学, 医学部, 講師 (60226496)
須田 治彦  都立保健科学大学, 保健学科, 教授 (40051784)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 2002: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 2001: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywordspost-transcriptional regulation / GIRK2 / AU-rich element / RNA binding protein / cloning / brain / rat / Down syndrome / post-trnscriptional regulation / differential display / G protein coupled inwardly rectifying channel / adenylate uridylate rich element
Research Abstract

GIRK2 (G protein-gated inwardly rectifying K(+) channel 2) located on the Down syndrome region 21q22.2 in humans has been reported to have several alternative transcripts and transcripts longer than 4 kb that do not have the poly-A tail. We sequenced GIRK2 transcripts with a long 3'-untranslated region (3'-UTR) containing multiple adenylate uridylate-rich elements (AREs) with the poly-A tail. In a 16-kb transcript, 28 AUUUA pentanucleotides, 9 AUUUUA hexanucleotides, 5 AUUUUUA heptanucleotides, and 3 UUAUUUA[U/A] [U/A] nonanucleotides were found. Northern blot and in situ hybridization revealed abundant expression of the 16-kb transcripts in the rat brain despite no detectable signals in other tissues examined. The AREs have been reported to mediate the turnover of mRNAs encoding proteins regulating cellular proliferation/differentiation and body response to inflammatory and environmental stimuli. This is the first study indicating that ion channel transcripts have multiple AREs.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Suda S, Nibuya M, Suda H, Takamatsu K, Miyazaki T, Nomura S, Kawai N: "Potassium channel mRNA with AU-rich elements and brain specific expression"Biochem Biophys Res Commun. 291. 1265-1271 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nibuya M, Sugiyama H, Shioda K, Nakamura K, Nishjima K: "Psychiatric symptoms in Basedow's disease successfully treated with electroconvulsive therapy"J ECT. 18. 54-57 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nishijima K, Nibuya H, Kato S: "Toxic setoronin syndrome successfully treated with electroconvulsive therapy"J Clin Psychopharmacology. 22. 338-339 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 丹生谷正史, 須田史朗, 野村総一郎: "転写物3'非翻訳領域による転写後調節機構"神経精神薬理学雑誌. (印刷中). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nishijima K, Nibuya H, Kato S: "Monoamine and indolamine metabolites in CSF of serotonergic syndrome"J Clin Psychopharmacology. (印刷中). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 松下正明 編: "精神医学-その基盤と進歩"朝倉書店. 499 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Suda S, Nibuya M, Suda H, Takamatsu K, Miyazaki T, Nomura S, Kawai N: "Potassium channel mRNA with AU-rich elements and brain specific expression"Biochem Biophys Res Commun. 291. 1265-1271 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nibuya M, Sugiyama E, Shioda K, Nakamura K, Nishijima K: "Psychiatric symptoms in Basedow's disease successfully treated with electroconvulsive therapy"J ECT. 18. 54-57 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nishijima K, Nibuya M, Kato S: "Toxic serotonin syndrome successfully treated with electroconvulsive therapy"J Clin Psychopharmacology. 22. 338-339 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nibuya M, Suda S, Nomura S: "Post-transcriptional regulation by 3'-untranslated region of transcripts"Jpn J Neuropsychopharmacol. (in press). (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Suda, s., Nibuya, M., Suda, H., Takamatu, K., Miyazaku, T., Nomura, S., Kawai, N.: "Potassium channel mRNA with AU-rich elements and brain specific expression"Biochem. Biophys. Res. Commun.. 291. 1265-1271 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nibuya, M., Sugiyama, H., Shioda, K., Nakamura, K., Nishijima, K.: "Psychiatric symptoms in Basedow's disease successfully treated with electroconvulsive therapy"J. ECT. 18. 54-57 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nishijima, K., Nibuya, M., Kato, S.: "Toxic serotonin syndrom successfully treated with elecroconvulsive therapy"J. Clin. Psychopharmacology. 22. 338-339 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 丹生谷正史, 須田史朗, 野村総一郎: "転写物3'非翻訳領域による転写後調節機構"神経精神薬理雑誌. (印刷中). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nishijima, K., Nibuya, M., Kato, S.: "Monoamine and indolamine metabolites in CSF of serotonergic syndrome"J. Clic. Psychopharmacology. (印刷中). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 松下正明 編: "精神医学-その基盤と進歩"朝倉書店. 499 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Suda S, ^*Nibuya M, Suda H, Takamatsu K, Miyazaki T, Nomura S, Kawai N: "Potassium channel mRNA with AU-rich elements and brain specific expression"Biochem Biophys Res Commun.. 291. 1265-1271 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nibuya M, Sugiyama H, Shioda K, Nakamura K, Nishijima K: "Psychiatric symptoms in Basedow's disease Successfully treated with electro convulsive therapy"J ECT. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Nishijima K, Nibuya M, Kato S: "Toxic setoronin syndrome successfully treated with electro convulsive therapy"J Clin Psychopharmacol. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] 丹生谷正史, 川合述史: "精神医学(松下正明編、精神科専門医制度を想定した、精神科医用の全般的教科書)の中の1章神経科学・神経生理"中山書店、東京. (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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