| Project/Area Number |
13671048
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | KYOTO UNIVERSITY (2002)
The University of Tokyo (2001) |
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Principal Investigator |
MAEKAWA Taira Departments of Transfusion Medicine and Cell Therapy, Professor of Medicine, 医学研究科, 教授 (80229286)
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| Co-Investigator(Kenkyū-buntansha) |
TSUJI Kouichiro Department of Pediatrics, The Institute of Medical Science, University of Tokyo, Associate Professor, 医科学研究所, 助教授 (50179991)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2001: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | cord blood transplantation / hematopietic stem cell / chemokine / homing |
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| Research Abstract |
We found that the stromal cell derived factor-1/pre-B cell growth stimulating factor (SDF1/PBSF) receptor, CXCR4, is expressed on human CD34^+ bone marrow (BM) cells. Stringently FACS-sorted CD34^+CXCR4^+ BM cells completely lack myeloid, erythroid, megakaryocytic, and mixed colony forming potential (myeloid progenitors), but give rise to B and T lymphoid progenitors, whereas CD34^+CXCR4^- BM cells can generate colonies formed by myeloid progenitors and can also develop into these lymphoid progenitors. Therefore, expression of CXCR4 on CD34^+ BM cells can allow lymphoid progenitors to be discriminated from myeloid progenitors. Since CD34^+CXCR4^+ cells are differentiated from CD34^+CXCR4^- cells, multipotential progenitors located in the BM are likely to be negative for CXCR4 expression. CXCR4 seems to be expressed earlier than the interleukin-7 receptor and terminal deoxynucleotidyl transferase during early lymphohemopoiesis. These results suggest that the expression of CXCR4 on CD34^+ BM cells is one of the phenotypic alterations for committed lymphoid progenitors.
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