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The Analysis of Signal Transduction System in the Activation of Proplatelet Formation of Megakaryocytes

Research Project

Project/Area Number 13671075
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Hematology
Research InstitutionIwate Medical University

Principal Investigator

ISHIDA Yoji  Iwate Medical Univ. School of Med. Associate Prof, 医学部, 助教授 (70151389)

Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2002: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2001: ¥1,900,000 (Direct Cost: ¥1,900,000)
Keywordsproplatelet formation / platelet production / AT III / HDL / protein kinase C / Rho kinase / prplatelet formation / 巨核球胞体突起形成(PPF) / PPF刺激因子 / 巨核芽球性白血病細胞株 / アンチトロンビンIII / トロンビン
Research Abstract

1. Purification of proplatelet formation (PPF) stimulatory factor.
We purified the PPF stimulatory factor from the normal human plasma and determined the amino acid sequence of the purified protein. It was antithrombin III, which in vitro activity expressed in association with high density lipoprotein (HDL).
2. Selection of human megakaryoblastic cell line, which respond to PPF stimulatory factor.
We established the quantitative assay system in which MEG cell line expressed PPF in response to AT III/HDL. AT III/HDL stimulated PPF of MEG cell line in a dose dependent manner (HDL 0 μg/ml 1.1%, HDL 50 μg/ml 30.8%, HDL 100 μg/ml 46.5%, HDL 200 μg/ml 57.8%).
3. Inhibition of PPF expression or not in the above assay system with the incubation of kinase inhibitors. PD98059(MAPK inhibitor), LY294002(PI3K inhibitor), SB203580(p38 MAPK inhibitor), KT5720(PKA inhibitor), BIM(PKC activator inhibitor) or Y27632(Rho kinase inhibitor) was added in the above assay system. PPF expression of MEG cell line was inhibited by the addition of BIM or Y27632 (BIM : 100 μM 70.7%, 500 nM 66.9%, 1 μM 54.0%, 10 μM 4.5%, 100 μM 5.6%, Y27632 :100 nM 97.6%, 1 μM 88.5%, 10 μM 61.3%).
Based on the results, PPF expression of megakaryocytes was deeply involved in the transduction systems of protein kinase C and Rho kinase.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Yoji Ishida: "Purification of proplatelet formation (PPF) stimulating factor : Thrombin/anti-thrombin III complex stimulates PPF of megakaryocytes in vitro and platelet production in vivo"Thrombosis & Haemostasis. 85. 349-355 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shigeki Ito: "Flow cytometric analysis of aberrant antigen expression of blasts using CD45 blast gating for minimal residual disease in acute leukemia and high-risk myelodysplastic syndrome"Leukemia Research. 25. 205-211 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kiyoyuki Ogata: "Reappraisal of the clinical significance of CD7 expression in association with cytogenetics in de novo acute myeloid leukaemia"British Journal Haematology. 115. 945-948 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shigeki Ito: "Clonal evolution of blasts in an elderly patient with CD56+ relapsed acute promyelocytic leukemia"American Journal of Hematology. 69. 59-63 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shie Ohgi: "Spontaneous regression of malignant lymphoma of the lumbar spine"Skeletal Radiol. 31. 99-102 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yoji Ishida: "Rapid Cycle Real-Time PCR Methods and Applications"S Meuer, C Wittwer, K Nakagawara (Springer). 408 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yoji Ishida, Kazuo Yano, Toshiharu Ito, Hiroki Shigematus, Kohsuke Sasaki, Shuhei Kondo, Shin-Ichiro Kuriya: "Purification of proplatelet formation (PPF) stimulating factor : Thrombin/antithrombin III complex stimulates PPF of megakaryocytes in vitro and platelet production in vivo"Thrombosis & Haemostasis. 85. 349-355 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shigeki Ito, Yoji Ishida, Kazunori Murai, Shinichiro Kuriya: "Flow cytometric analysis of aberrant antigen expression of blasts using CD45 blast gating for minimal residual dis-ease in acute leukemia and high-risk myelodysplastic syndrome"Leukemia Research. 25(3). 205-211 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Ogata, N.Yokose, Y.Shioi, Y.Ishida, J.Tomiyama, H.Hamaguchi, F.Yagasaki, M.Bessyo, H.Sakamaki, K.Dan and S.Kuriya: "Reappraisal of the clinical significance of CD7 expression in association with cytogenetics in de novo acute myeloid leukemia"Brit J Haematol. 115. 612-615 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shigeki Itoh, Takeshi Sugawara, Sanae Enomoto, Yoko Ono, Hideharu Numaoka, Taiju Utsugisawa, Kazunori Murai, Yoji Ishida Y.Shin-ichiro Kuriya: "Clonal evolution of blasts in an elderly patient with CD56 + relapsed acute promyelocytic leukemia"American Journal of Hematology. 69. 59-63 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shie Ohgi, Shigeru Ehara, Takashi Satoh, Sadafumi Kato, Kenji Shimosegawa, Yoji Ishida: "Spontaneous regression of malignant lymphoma of the lumbar spine"Skeletal Radiol. 31. 99-102 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yoji Ishida, Kazunori Murai, eds : S.Meuer, G.Wittwer and K.Nakagawara: "Development of quantitative RT-PCR for the expression of Wilms' tumor in leukemia on the Light Cycler System"Rapid Cycle Real Time PCR-Methods and Applications Springer (Germany). 187-195 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shigeki Ito: "Flow cytometric analysis of aberrant expression of blasts using CD45 blast gating for minimal residual disease in acute leukemia and high-risk myelodysplastic syndrome"Leukemia Research. 25. 205-211 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kiyokuni Ogata: "Reappraisal of the clinical significance of CD7 expression in association with cytogenetics in de novo acute myeloid leukemia"British Journal Haematology. 115. 945-948 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shigeki Ito: "Clonal evolution of blasts in an elderly patient with CD56+ relapsed acute promyelocytic leukemia"American Journal of Hematology. 69. 59-63 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shie Ohgi: "Spontaneous regression of malignant lymphoma of the lumbar spine"Skeletal Radiol. 31. 99-102 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 石田 陽治: "EBM血液疾患の治療"押味和夫, 別所正美, 岡本真一郎, 加藤淳. 739 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yoji Ishida: "Purification of proplatelet formation (PPF) Stimulating factor : Thrombin/antithrombin III complex stimulates PPF of megakaryocytes in vitro and platelet production in vivo"Thrombosis & Haemostasis. 85. 349-355 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Shigeki Ito: "Flow cytometric analysis of aberrant antigen expression of blasts using CD45 blast gating for minimal residual disease in acute leukemia and high-risk myelodysplastic syndrome"Leukemia Research. 25. 205-211 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Shigeki Ito: "Clonal evolution of blasts man elderly patient with CD56+ relapsed acute promyelocytic leukemia"American Journal of Hematology. (In press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Koichi Ogata: "Reappraisal of the clinical significance of CD7 expression in association with cytogenetics in de novo acute myeloid leukaemia"Brit. J. Haematol. (In press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yoji Ishida: "Rapid Cycle Real Time PCR-Methods and Applications eds : Meuer, Wittwer and K. Nakagawara)"Development of quantitative RT-PCR for the expression of Wilms' tumor in leukemia on the LightCycler System. 187-195 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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