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Analysis of onset mechanism in refractory glomerulonephritis with DNA microanray

Research Project

Project/Area Number 13671104
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Kidney internal medicine
Research InstitutionNiigata University

Principal Investigator

UENO Mitsuhiro  NIIGATA UNIVERSITY, Medical and dental hospital, Lecturer, 医歯学総合病院, 講師 (90260546)

Co-Investigator(Kenkyū-buntansha) GEJYO Fumitake  NIIGATA UNIVERSITY, Medical and dental hospital, Professor, 医歯学総合病院, 教授 (20126410)
SAKATSUME Minoru  NIIGATA UNIVERSITY, Medical and dental hospital, Assistant, 医歯学総合病院, 助手 (70334662)
NARITA Ichiei  NIIGATA UNIVERSITY, Graduate school of medical and dental sciences, Associate Professor, 大学院・医歯学総合研究科, 助教授 (20272817)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2003: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 2002: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2001: ¥2,300,000 (Direct Cost: ¥2,300,000)
Keywordsrefractory glomerulonephritis / IgA nephropathy / crescentic glomerulonephritis / single nucleotide polymorphism / DNA microarray / macrophage metalloelastase / aldosterone Synthase / α addducin / 高血圧 / Macrophage mettalloelastase / α-adducin / アンジオテンシノーゲン / CYP11B2 / FcR受容体 / 多価免疫受容体 / ウテログロビン / DNAマイクロアレイ解析 / クラスタリング / 腎生検 / ネフローゼ症候群
Research Abstract

Both IgA nephropathy and crescentic glomerulonephritis are typical of refractory glomerulonephritis. The precise onset mechanisms of both glomerulonephtitis are unknown. However genetical factors may be associated with the onset of IgA nephropathy because of familial clustering. We attempted to investigate the possible role of various gene polymorphisms in IgA nephropathy and studied any possible associations between gene variation and the clinical manifestations, especially renal prognosis in about 250 patients. As a result, we demonstrated that single nucleotide polymorphisms of polymeric immunoglobulin receptor, uteroglobin, angiotensin converting enzyme angiotensinogen aldosterone synthase, SA, α addducin and peroxisome proliferator-activated receptor γ were related to renal prognosis of IgA nephropathy.
Rat anti-glomerular basement membrane(GBM) nephritis is a model of crescentic glomerulonepnntis induced by the administration of anti-GBM antiserum. To elucidate the mechanism of gl … More omerular injury, we analyzed the gene expression patterns in the kidneys of anti-GBM nephritis rats using DNA arrays, and found that macropgage metalloelastase/matrix metalloproteinase(MMP)-12 was lone of the highly expressed genes in the kidneys on days 3 and 7 after the injection of anti-GBM antiserum. Enhancement of MMP-12 mRNA expression was confirmed by Northern blot analysis, and in situ hybridization revealed that MMP-12 mRNA was expressed in ED-1-positive macrophages and multinuclear giant cells in the glomeruli with crescent. Moreover, these cells were positive with anti-rat rMMP-12 Ab on the section of the kidneys of anti-GBM nephritis rats on day 7. To clarify the role of MMP-12, we conducted a neutralization experiment using anti-rat rMMP-12 Ab. Consequently, crescent formation and macrophage infiltration in the glomeruli were significantly rethiced in the rats treated with anti-rat rMMP-12 Ab, and the amount of urine protein was also decreased. These results disclosed that MMP-12 played an important role in glomerular injury in a crescentic glomerulonephritis model. Less

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] Kaneko Y, et al.: "Macrophage metalloelastase as a major factor for glomerular injury in anti-glomerular basement membrane nephritis"Journal of immunology. 170(6). 3377-3386 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Song Jin, et al.: "Gender Specific Association of Aldosterone Synthase Gene Polymorphism with Renal Survival in Patients with IgA Nephropathy"Journal of Medical Genetics. 40(5). 372-376 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Song Jin, et al.: "Peroxisome proliferator-activated receptor γ C161T polymorphisms and survival of Japanese patients with immunoglobulin A nephropathy"Clinical Genetics. 64(5). 398-403 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Narita Ichiei et al.: "Role of uteroglobin G38A polymorphism in the progression of IgA nephropathy in Japanese patients"Kidney International. 61(5). 1853-1858 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Goto Shin, et al.: "A(-20)C polymorphism of the angiotensinogen gene and progression of IgA nephropathy"Kidney International. 62(3). 980-985 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Narita Ichiei et al.: "Role of genetic polymorphism in the SA gene on the blood pressure and prognosis of renal function in patients with immunoglobulin A nephropathy."Hypertension Research. 25(6). 831-836 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ichiei Naeita, et al.: "Interaction between ACE and ADD1 gene polymorphisms in the progression of IgA nephropathy in Japanese patients."Hypertension. 42(3). 304-309 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoshikatsu Kaneko, et al.: "Macrophage metalloelastase as a major factor for glomerular injury in anti-glomerular basement membrane nephritis."Journal of immunology. 170(6). 3377-3386 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Jin Song, et al.: "Gender specific association of aldosterone synthase gene polymorphism with renal survival in patients with IgA nephropathy."Journal of Medical Genetics. 40(5). 372-376 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Jin Song, et al.: "Peroxisome proliferatoractivated receptor y C161T polymorphisms and survival of Japanese patients with immunoglobulin A nephropathy."Clinical Genetics. 64(5). 398-403 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ichiei Naeita, et al.: "Role of uteroglobin G38A polymorphism in the progression of IgA nephropathy in Japanese patients"Kidney International. 61(5). 1853-1858 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shin Goto, et al.: "A(-20)C polymorphism of the angiotensinogen gene and progression of IgA nephropathy"Kidney International. 62(3). 980-985 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ichiei Naeita, et al.: "Role of genetic polymorphism in the SA gene on the blood pressure and prognosis of renal function in patients with immunoglobulin A nephropathy."Hypertension Research. 25(6). 831-836 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ichiei Naeita, et al.: "Association of gene polymorphism of polymeric immunoglobulin receptor and IgA nephropathy."Internal Medicine. 40(9). 867-872 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ichiei Naeita, et al.: "Genetic polymorphisms in the promoter and 5' UTR region of the Fc alphareceptor(CD89) are not associated with a risk of IgA nephropathy."Journal of Human Genetics. 46. 694-698 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ichiei Naeita, et al.: "Genetic polymorphism in angiotensinogen prompter region affects progression of IgA nephropathy."Nephrology. 6 Supple. A26-A27 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Xie Y., et al.: "The efficacy of tonsillectomy on long-term renal survival in patients with Iga nephropathy"Kidney International. 63(5). 1861-1867 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kaneko Y, et al.: "Macrophage metalloelastase as a major factor for glomerular injury in anti-glomerular basement membrane nephritis"Journal of immunology. 170(6). 3377-3386 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Song Jin, et al.: "Gender Specific Association of Aldosterone Synthase Gene Polymorphism with Renal Survival in Patients with IgA Nephropathy"Journal of Medical Genetics. 40(5). 372-376 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Narita I, et al.: "Angiotensinogen gene variation and renoprotective efficacy of rennin-angiotensin system blockade in IgA nephropathy"Kidney International. 64(3). 1050-1058 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Narita I, et al.: "Interaction between ACE and ADD1 gene polymorphisms in the progression of IgA nephropathy in Japanese patients"Hypertension. 42(3). 304-309 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Song Jin, et al.: "Peroxisome proliferator-activated receptor γ C161T polymorphisms and survival of Japanese patients with immunoglobulin A nephropathy"Clinical Genetics. 64(5). 398-403 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Song Jin, et al.: "Gender Specific Association of Aldosterone Synthase Gene Polymorphism with Renal Survival in Patients with IgA Nephropathy"Journal of Medical Genetics. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Xie, Y., et al.: "The efficacy of tonsillectomy on long-term renal survival in patients with IgA nephropathy"Kidney international. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Narita Ichiei, et al.: "Role of genetic polymorphism in the SA gene on the blood pressure and prognosis of renal function in patients with IgA nephropathy"Hypertension Research. 25. 831-836 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Narita Ichiei, et al.: "Role of uteroglobin G38A polymorphism in the progression of IgA nephropathy in Japanese patients"Kidney international. 61. 1853-1858 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Goto Shin, et al.: "A(-20)C polymorphism of the angiotensinogen gene and progression of IgA nephropathy"Kidney international. 62. 980-985 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yuansheng Xie: "Expression of osteopontin in gentamycin-induced acute tubular necrosis and its recovery phase"Kidney International. 60. 1645-1657 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ichiei Narita: "Role of uteroglobin G38A polymorphism in progressin of IgA nephropathy in Japanese patients"Kidney International. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Ichiei Narita: "Genetic plymorphisms in the promoter and 5' UTR region of the Fc alpha receptor (CD89) are not associated with a risk of IgA nephropathy"Journal of Human Genetics. 44. 694-698 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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