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Effects of gene transfer with inhibitory SMADs on experimental nephritis.

Research Project

Project/Area Number 13671123
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Kidney internal medicine
Research InstitutionOSAKA CITY UNIVERSITY

Principal Investigator

NEGOR Nobuo  OSAKA CITY UNIVERSITY, GRADUATE SCHOOL OF MEDICINE, RESEARCH ASSOCIATE, 大学院・医学研究科, 助手 (80180701)

Co-Investigator(Kenkyū-buntansha) OKAMURA Mikio  OSAKA CITY UNIVERSITY, GRADUATE SCHOOL OF MEDICINE, ASSISTANT PROFESSOR, 大学院・医学研究科, 講師 (90169144)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2001: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordstransforming growth factor β / cell culture / gene transfer / glomerulonephritis / SMAD
Research Abstract

Effects of gene transfer with inhibitory SMADs on experimental nephritis.
1) SMAD7 cDNA was cloned from human monocytic cell line THP-1 cells by reverse-transcriptation polymerase chain reaction (RT-PCR).
2) The SMAD7 cDNA was transferred to expression vector with 6 x His tag.
3) The expression vector with SMAD7 and 6 x His tag was transferred into adenovius medicated expression vector and prepared high concentration of recombinant adenovirus by using 293 cells.
4) The proliferation and product ion of extracellular matrix and TGF-β in cultured rat mesangial cells were inhibited by gene transfer of the SMAD7 gene.
5) The SMAD7 gene transfer inhibited the extracellular matrix and TGF-β stained with their specific antibodies in the mesangial cells in Thy-1 glomerulonephritis rats, but it did not inhibit the cell proliration marker (PCNA) stained with the antibodies in the rats.
6) The SMAD7 gene transfer did not inhibit the extracellular matrix and TGF-β and PCNA stained with their antibodies in IgA nephritis model mice.
These results suggest that the effects of the gene transfer with inhibitory SMAD (SMAD7) on the experimental models of nephritis may be different from the kinds of animal models of nephritis in vivo.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] 岡崎瑞江: "ヒト由来メサンギウム細胞の増殖および基質産生に対するトラニストの影響"大阪市医学会誌. 第51巻 第3・4号. 103-110 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Mizue Okazaki: "Tranilast inhibits cell proliferation and collangen sysnthesis in human mesangial cells."The J Osaka City Medical Center. Vol. 51, No. 3. 4 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 岡崎瑞江: "ヒト由来メサンギウム細胞の増殖および基質産生に対するトラニストの影響"大阪市医学会誌. 第51巻第3・4号. 103-110 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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