| Project/Area Number |
13671123
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Kidney internal medicine
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| Research Institution | OSAKA CITY UNIVERSITY |
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Principal Investigator |
NEGOR Nobuo OSAKA CITY UNIVERSITY, GRADUATE SCHOOL OF MEDICINE, RESEARCH ASSOCIATE, 大学院・医学研究科, 助手 (80180701)
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| Co-Investigator(Kenkyū-buntansha) |
OKAMURA Mikio OSAKA CITY UNIVERSITY, GRADUATE SCHOOL OF MEDICINE, ASSISTANT PROFESSOR, 大学院・医学研究科, 講師 (90169144)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2001: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | transforming growth factor β / cell culture / gene transfer / glomerulonephritis / SMAD |
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| Research Abstract |
Effects of gene transfer with inhibitory SMADs on experimental nephritis.
1) SMAD7 cDNA was cloned from human monocytic cell line THP-1 cells by reverse-transcriptation polymerase chain reaction (RT-PCR).
2) The SMAD7 cDNA was transferred to expression vector with 6 x His tag.
3) The expression vector with SMAD7 and 6 x His tag was transferred into adenovius medicated expression vector and prepared high concentration of recombinant adenovirus by using 293 cells.
4) The proliferation and product ion of extracellular matrix and TGF-β in cultured rat mesangial cells were inhibited by gene transfer of the SMAD7 gene.
5) The SMAD7 gene transfer inhibited the extracellular matrix and TGF-β stained with their specific antibodies in the mesangial cells in Thy-1 glomerulonephritis rats, but it did not inhibit the cell proliration marker (PCNA) stained with the antibodies in the rats.
6) The SMAD7 gene transfer did not inhibit the extracellular matrix and TGF-β and PCNA stained with their antibodies in IgA nephritis model mice.
These results suggest that the effects of the gene transfer with inhibitory SMAD (SMAD7) on the experimental models of nephritis may be different from the kinds of animal models of nephritis in vivo.
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