• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

A Study on the metabolism and action of thyroid hormones in the bone and cartilage.

Research Project

Project/Area Number 13671166
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Endocrinology
Research InstitutionKoshien University

Principal Investigator

TANAKA Kiyoshi  Koshien University, College of Nutrition, Professor, 栄養学部, 教授 (90227132)

Co-Investigator(Kenkyū-buntansha) TAGAMI Tetsuya  National Kyoto Hospital, Department of Clinical Studies, Physician, 臨床研究部, 医師 (60273439)
OGAWA Yoshihiro  Kyoto University, Faculty of Medicine, Assistant Professor, 大学院・医学研究科, 助手 (70291424)
ORITA Yoshimasa  Koshien University, College of Nutrition, Professor, 栄養学部, 教授 (70028398)
UMEZAWA Makiko  Koshien University, College of Nutrition, Associate Professor, 栄養学部, 助教授 (80231182)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2001: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywordsthyroid hormone / deiodinase / growth plate / osteoblast / osteoclast / vitamin D / ビタミンD3 / クレチン症 / ATDC5細胞
Research Abstract

Thyroid hormone deficiency is one of the important causes of dwarfism. The underlying mechanism, however, remains to be elucidated. We then studied the thyroid hormone effect on growth plate cartilage with emphasis on the metabolism. Thyroxin (T4) must be converted to 3,5,3,-triiodothyronine (T3) catalyzed by 5'-deiodinase for its action. We have found that 5'-deiodinase is present in ATDC-5 cell that is a good model to study the cartilage development. Both T4 and T3 enhanced the differentiation markers of cartilage. When thyroid hormones were added to the organ culture system, they markedly stimulated the longitudinal bone growth. Iopanoic acid, an inhibitor of 5'-deiodinase, abolished T4 action without affecting T3 action.
We also studied the bone effects of thyroid hormones on the bone, since thyroid hormone excess is known to be an important cause of secondary osteoporosis with increased bone resorption. 5'-deiodinase was present in primary-cultured osteoblast. Thyroid hormones enhanced the expression of RANKL that is a key molecule in osteoclast formation. Although thyroid hormones alone did not induce osteoclast formation, they markedly enhanced the vitamin D-induced osteoclast formation. Thus a novel interaction between thyroid hormones and vitamin D in osteoclast formation was suggested.
In this study, we have shown the importance of local activation of thyroid hormones for their skeletal effects.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (8 results)

All 2002 Other

All Journal Article (4 results) Publications (4 results)

  • [Journal Article] Thyroid hormones promote chondrocyte differentiation in mouse ATDC 5 cells and stimulate endochondral ossification in fetal muse tibias through iodothyronine deiodinause in the growth plate.2002

    • Author(s)
      Miura M
    • Journal Title

      Journal of Bone and Mineral Research 17

      Pages: 443-454

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] A novel interaction between thyroid hormones and 1,25(OH)2D3 in osteoclast formation.2002

    • Author(s)
      Miura M
    • Journal Title

      Biochemical and Biophysical Research Communication 291

      Pages: 987-994

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Thyroid hormones promote chondrocyte differentiation in mouse ATDC5 cells and stimulate endochondral ossification in fetal mouse tibias through iodothyronine deiodinases in the growth plate.2002

    • Author(s)
      Miura M, Tanaka K et al.
    • Journal Title

      Journal of Bone and Mineral Research 17

      Pages: 443-454

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] A novel interaction between thyroid hormones and 1,25(OH)2D3 in osteoclast formation.2002

    • Author(s)
      Miura M, Tanaka K et al.
    • Journal Title

      Biochemical and Biophysical Research Communication 291

      Pages: 987-994

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 三浦晶子, 田中清 (他): "Thyroid hormones promote chondrocyte differentiation in mouse ATDC5 cells and stimulate endochondral ossification in fetal mouse tibias through iodothyronine deiodinases in the growth plate"J Bone and Mineral Res. 17(3). 443-454 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 三浦晶子, 田中清 (他): "A novel interaction between thyroid hormones and 1,25(OH)_2D_3 in Osteoclast formation"Biochem Biophys Res Commun. 291. 987-994 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Miura A., Tanaka K et al.: "Thyroid Hormones Promote Chondrocyte Differentiation in Mouse ATDC5 Cells and Stimulate Endochondral Ossification in Fetal Mouse Tibiae through lodothyronine Deiodinases in the Growth Plate"J Bone Miner Res. (印刷中). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Miura A., Tanaka K et al.: "A novel interaction between thyroid hormones and 1,25(OH)_2D_3 in osteoclast formation"Bilchem Bilphys Res Commun. (印刷中). (2002)

    • Related Report
      2001 Annual Research Report

URL: 

Published: 2001-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi