• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

EXPRESSION OF MUCOSAL ADDRESSIN CELL ADHESION MOLECULE- (MAdCAM-1) DURING SMALL BOWEL GRAFT REJECTION IN RATS

Research Project

Project/Area Number 13671259
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionNihon University

Principal Investigator

FUJISAKI Shigeru  Nihon University School of Medicine, Assistant Professor, 医学部, 講師 (60287638)

Co-Investigator(Kenkyū-buntansha) SUGITOH Kiminobu  Nihon University School of Medicine, Assistant Professor, 医学部, 助手 (10328750)
JI PARK Yeong  Nihon University School of Medicine, Assistant Professor, 医学部, 助手 (70318426)
FUKUZAWA Masahiro  Nihon University School of Medicine, Professor, 医学部, 教授 (60165272)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2002: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2001: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsSmall bowel transplantation / Rejection / MAdCAM-1 / Rat / Peyer's patch / パイエル板
Research Abstract

Background. Mucosal addressin cell adhesion molecule-1 (MAdCAM-1) is a critical endothelial adhesion molecule for lymphocyte trafficking to gut-associated lymphoid tissues (GAULT) under physiological conditions and it is expressed on special postcapillary venules, the high endothelial venules (HEV) in GALT. In this study, we investigated changes of MAdCAM-1 expression during small bowel graft rejection.
Methods. Orthotopic small bowel transplantation (SBT) with portocaval drainage was performed from Brown Norway (BN) rats to Lewis (LEW) rats. Isografted (LEW→LEW) and untransplanted animals served as control. Animals were killed on days 3, 4, 5, 6 and 7 after SBT. Six-mm-thick cryostat sections were prepared from normal small bowel tissues and small bowel grafts, including Peyer's patches (PPs). Indirect immunoperoxidase staining was performed using monoclonal antibodies (mAbs) against rat MAdCAM-1 (OST12).
Results. In the PPs of controls, MAdCAM-1 antibodies specifically stained the endothelial cells of HEV, which were predominantly located, in the interfollicular areas (IFA). In the allografts on day 4 after SBT, the MAdCAM-1 expression was weaker on the HEV in the PPs than controls. In the lamina propria of controls, the faint expression of MAdCAM-1 on vessels was observed. On day 4 after allogeneic SBT, the MAdCAM-1 was more strongly expressed on the endothelial cells of the vessels at the base of the villi. As rejection developed, the MAdCAM-1 expression on the vessels progressed toward the villus tip.
Conclusions. The change of MAdCAM-1 expression may be involved in the development of small bowel graft rejection. The vessels at the base of villi, which is associated with lymphocyte recruitment, may become a site of intense immune reactivity on the early phase of small bowel allograft rejection.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Fujisaki S, Park YJ, Kimizuka K, et al.: "Expression of mucosal addressin cell adhesion molecule-1(MAdCAM-1)during small bowel graft rejection in rats"Scandinavian Journal of Gastroenterology. 38(4). 437-442 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 藤崎 滋, 君塚 圭, 朴 英智, 他: "小腸移植拒絶反応における腸管粘膜防御機構の破綻-特にPeyer板の変化に関して-"Digestion & Absorption. 25(1). 55-59 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 藤崎 滋, 君塚 圭, 朴 英智, 他: "小腸移植拒絶反応における粘膜防御機構の破綻"消化器科. 35. 494-499 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Fujisaki S, Park YJ, Kimizuka K, Inoue M, Tomita R, Fukuzawa M, Matsumoto K.: "Expression of mucosal addressin cell adhesion molecule-1 (MAdCAM-1) during small bowel graft rejection in rats"Scandinavian Journal of Gastroenterology. 38 (4). 437-442 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Fujisaki S, Park YJ, Kimizuka K, et al.: "Expression of mucosal addressin cell adhesion molecule-1 (MAdCAM-1) during small bowel graft rejection in rats"Scandinavian Journal of Gastroenterology. 38(in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 藤崎 滋, 君塚 圭, 朴 英智, 他: "小腸移植拒絶反応における腸管粘膜防御機構の破綻-特にPeyer板の変化に関して-"Digestion & Absorption. 25・1. 55-59 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 藤崎 滋, 君塚 圭, 朴 英智, 他: "小腸移植拒絶反応における粘膜防御機構の破綻"消化器科. 35. 494-499 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Fujisaki S, et al: "IMMUNOHISTOCHEMICAL ANALYSIS OF MAdCAM-1 EXPRESSION DURING ACUTE REJECTION ON SMALL BOWEL GRAFTS IN RATS"Transplantation Proceedings. (印刷中). (2002)

    • Related Report
      2001 Annual Research Report

URL: 

Published: 2001-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi