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Immunological analysis of micro lymph node metastasis in gastrointestinal cancer and its clinical implication

Research Project

Project/Area Number 13671314
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionKagoshima University

Principal Investigator

ISHIGAMI Sumiya  Kagoshima University, University Hospital, Faculty of Medicine, Research Associate, 医学部附属病院, 助手 (90325803)

Co-Investigator(Kenkyū-buntansha) TAKAO Sonshin  Kagoshima University, Faculty of Medicine, Associate Professor, 医学部, 助教授 (80171411)
AIKOU Takashi  Kagoshima University, Faculty of Medicine, Professor, 医学部, 教授 (60117471)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2002: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2001: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsLymph node / micrometastasis / immunology / Mouse model / CAM-1 / surgery / 腫瘍免疫
Research Abstract

Melanoma cell line B17F1 and HLA-matched mouse C57BL was used for this experiment. 5×106 B17F1 tumor cells were inoculated into food pad of C57BL mouse. The inoculated tumor proliferated exponentially, all of the mouse were died two months after tumor inoculation. The B17F1 tumor did not showed neither hematogeneous (liver, lung) nor peritoneal metastasis.
Approximately 34% of the inoculated mouse showed lymph node metastasis at inguinal region 3 weeks after tumor inoculation. We tried transfect GFP gene into the tumor using lipofectin method and tried to visualize micro-metastatic lesion in the lymph node using GFP protein as biomarker of the tumor. However, transfection of GFP gene into the tumor cells was failed. Then we disclosed that the tumor cells were specifically stained by anti CAM-1 antibody. Single tumor metastasis in the lymph node was clarified by immunohistochemical staining of CAM-1. We try to identify the term of establishment of micro-lymph node metastasis.
The primary tumor is extirpated respective weeks (2,3 and 4 weeks) after tumor inoculation, in order to clarify the development of residual micro-metastatic lesion in the lymph node. During latest 2 years, we could not get conclusive result and this plane is ongoing now. Our final goal is clarifying the development of residual metastatic lesion of the lymph node after surgery and analyzing immunological circumstance of micrometastatic lymph node.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] SUMIYA ISHIGAMI: "Clinical and pathological features in T-cell gastric lymphoma associated with human T-cell leukemia virus type 1 (HTLV-1) in comparison with B-cell gastric lymphoma"Hepatogastroenterology. 49. 1734-1741 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] SUMIYA ISHIGAMI: "CD3-zetachain expression of intratumoral lymphocytes is closely related to survival in gastric carcinoma patients"Cancer. 94. 1437-1442 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] SUMIYA ISHIGAMI: "Invariant chain expression in gastric cancer"Cancer Letters. 168. 87-91 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] SUMIYA ISHIGAMI: "Clinical importance of preoperative carcinoembryonic antigen and carbohydrate antigen 19-9 levels in gastric cancer"J Clin Gastroenterol. 32. 41-44 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 石神純也: "胃癌所属リンパ節内の抗腫瘍リンパ球の浸潤に関する検討"リンパ学. 24. 13-16 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 石神純也: "sm胃癌の深達度亜分類の臨床的意義と問題点"日本消化器外科学会雑誌. 34. 445-448 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sumiya Ishigami: "Clinical and pathological features in T-cell gastric Lymphoma associated with human T-cell leukemia virus type 1 (HTLV-1) in comparison with B-cell gastric lymphoma"Hepatogastroenterology. 49. 1734-1741 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sumiya Ishigami: "CD3 zeta chain expression of intratumoral lymphocytes in closely related to survival in gastric cancer"Cancer. 94. 1437-1442 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sumiya Ishigami: "Invariant chain expression in gastric cancer"Cancer Letters. 168. 87-91 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sumiya Ishigami: "Clinical importance of preoperative carcinomembryonic antigen and carbohydrate antigen 19-9 levels in gastric cancer"J Clin Gastroenterol. 32. 41-44 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] SUMIYA ISHIGAMI: "Clinical and pathological features in T-cell gastric lymphoma associated with human T-cell leukemia virus type 1 (HTLV-1) in comparison with B-cell gastric lymphoma"Hepatogastroenterology. 49. 1734-1741 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] SUMIYA ISHIGAMI: "CD3-zetachain expression of intratumoral lymphocytes is closely related to survival in gastric carcinoma patients"Cancer. 94. 1437-1442 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] SUMIYA ISHIGAMI: "Invariant chain expression in gastric cancer"Cancer Letters. 168. 87-91 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] SUMIYA ISHIGAMI: "Clinical importance of preoperative carcinoembryonic antigen and carbohydrate antigen 19-9 levels in gastric cancer"J Clin Gastroenterol. 32. 41-44 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] 石神純也: "胃癌所属リンパ節内の抗腫瘍リンパ球の浸潤に関する検討"リンパ学. 24. 13-16 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] 石神純也: "sm胃癌の深達度亜分類の臨床的意義と問題点"日本消化器外科学会雑誌. 34. 445-448 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ishigami S: "Clinical importance of preoperative carcinoembryonic antigen and carbohydrate antigen 19-9 levels in gastric cancer"J Clin Gastroenterol. 32. 41-44 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ishigami S: "Invariant chain expression in gastric cancer"Cancer Lett. 168. 87-91 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 石神純也: "sm胃癌の深達度亜分類の臨床的意義の問題点"日消外会誌. 34. 445-448 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 石神純也: "胃癌所属リンパ節内の抗腫瘍リンパ球浸潤に関する検討"リンパ学. 24. 13-16 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 石神純也: "所属リンパ節内リンパ球および胃癌浸潤リンパ球におけるCD3ζ鎖発現"Biotherapy. 15. 172 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ishigami S: "CD3 zeta chain expression of intratumoral lymphocytes is closely related to survival in gastric cancer"Cancer. (in press). (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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