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IDENTIFICATION OF METASTATIC-RELATED GENES AND A NOVEL APPROACH FOR THE INHIBITION OF METASTASIS USING HUMAN BREAST CANCER CELL LINES

Research Project

Project/Area Number 13671317
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionSapporo Medical University

Principal Investigator

HATA Fumitake  SAPPORO MEDICAL UNIVERSITY, SCHOOL OF MEDICINE, ASSO.PROFESSOR, 医学部, 助手 (70291557)

Co-Investigator(Kenkyū-buntansha) HONMA Toshio  SAPPORO MEDICAL UNIVERSITY, SCHOOL OF MEDICINE, INSTRUCTOR, 医学部, 助手 (30315494)
OHMURA Tosei  SAPPORO MEDICAL UNIVERSITY, SCHOOL OF MEDICINE, INSTRUCTOR, 医学部, 助手 (30295349)
HIRATA Koichi  SAPPORO MEDICAL UNIVERSITY, SCHOOL OF MEDICINE, PROFESSOR, 医学部, 教授 (50136959)
NISHIMORI Hidefumi  SAPPORO MEDICAL UNIVERSITY, SCHOOL OF MEDICINE, INSTRUCTOR, 医学部, 助手 (70336402)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2003: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2001: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsBREAST CANCER / BONE METASTASIS / LUNG METASTASIS / LIVER METASTASIS / LYMPH NODE METASTASIS / ANIMAL MODEL / DNA CHIP / GENE EXPRESSION ANALYSIS
Research Abstract

First, many human breast cancer cell lines were purchased from JCRB. They were injected into the breast of mice to establish highly metastatic cell lines. Unfortunately, these parental cell lines had not metastasized to the liver, lymph nodes, and lungs. The cell lines were then injected into the spleen in a manner similar to that used in previous research involving the pancreas and gastric cancer cell lines. In order for the breast cancer cell lines to metastasize to the lymph nodes, lungs, and liver, more time was required. An in vivo selection procedure was used. A highly metastatic cell line to the liver, ZR-H4,(metastatic rate:80%) was established. It originated from the parental breast cancer cell line, ZR75-1,(metastatic rate:10%). In the study period, it was not possible to establish cell lines that were highly metastatic to the lungs, bone, and lymph nodes. ZR75-1 and ZR-H4 are being used in a differential gene expression analysis, which is being conducted with a DNA chip.
While establishing breast cancer cell liens that were highly metastatic, differential gene expression was analyzed with the use of pancreatic cancer cell lines. This was done to establish a successful system of gene expression analysis. Tens of genes were identified as candidates that might be related to pancreatic cancer metastasis(J.Exp.Clin.Cancer Res.,2003;22:421-42). More cell lines were used, and several genes were identified as specific to liver and peritoneal metastases in pancreatic cancer. Once cancer cell lines that are highly metastatic to the lungs, lymph nodes, bones, and breast are established, gene expression analysis using a DNA chip will follow. The data are preliminary ; however, the information obtained has potential for clarifying the mechanisms of breast cancer metastasis and for providing a timeframe for the inhibition of cancer metastasis.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Nishimori H, Yasoshima T, Hata F, et al.: "A Novel Nude Mouse Model of Liver Metastasis and Peritoneal Dissemination from the Same Human Pancreatic Cancer Line"Pancreas. 24. 242-250 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nomura H, Nishimori H, Yasoshima T, Hata F, et al.: "A new liver metastatic and peritoneal dissemination model established from the same human pancreatic cancer cell line : analysis using cDNA macroarray"Clinical & Experimental Metasis. 19. 391-399 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tanaka H, Hata F, Nishimori H, et al.: "Differential Gene Expression Screening Between Parental and Highly Metastatic Pancreatic Cancer Variants Using a DNA Microarray"J.Exp.Clin.Cancer Res.. 22. 421-427 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohno K, Hata F, Nishimori H, et al.: "Biological properties and differential gene expression profiles in established highly liver or peritoneal metastatic cell lines of human pancreatic cancer"J.Clin.Exp.Cancer Res.. 22. 623-631 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] F.Hata, H.Nishimori, T.Yasoshima et al.: "Profiling Analysis of Differential Gene Expression Between Hematogenous and Peritoneal Metastatic Sublines of Human Pancreatic Cancer Using DNA chip"J.Clin.Exp.Cancer Res.. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tanaka H, Hata F, Nishimori H, Honmou O, Yasoshima T, Nomura H, Nakajima F, Hirai H, Kamiguchi K, Hirohashi Y, Sato N, Denno R, Hirata K.: "Differential Gene Expression Screening Between Parental and Highly Metastatic Pancreatic Cancer Variants Using a DNA Microarray."J.Exp.Clin.Cancer Res.. 22. 421-427 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohno K, Hata F, Nishimori H, Nomura N, Yasoshima T, Nakajima F, Tanaka H, Kitagawa S, Hirai I, Kamiguchi K, Isomura H, Sato N, Denno R, Hirata K.: "Biological properties and differential gene expression profiles in established highly liver or peritoneal metastatic cell Lines of human pancreatic cancer."J.Clin.Exp.Can.Res.. 22. 623-631 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] F.Hata, H.Nishimori, T.Yasoshima, H.Tanaka, K.Ohno, Y.Yanai, E.Ezoe, K.Sogahata, K.Kamiguchi, H.Isomura, R.Denno, N.Sato, K.Hirata: "Profiling Analysis of Differential Gene Expression Between Hematogenous and Peritoneal Metastatic Sublines of Human Pancreatic Cancer Using DNA chip."J.Clin.Exp.Can.Res.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tanaka H, Hata F, Nishimori H, et al.: "Differential Gene Expression Screening Between Parental and Highly Metastatic Pancreatic Cancer Variants Using a DNA Microarray"J.Exp.Clin.Cancer Res.. 22. 421-427 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 西森 英史, 平田 公一: "動物モデルを用いた転移関連遺伝子群"医学のあゆみ. 266. 242-245 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ohno K, Hata F, Nishimori H, et al.: "Biological properties and differential gene expression profiles in established highly liver or peritoneal metastatic cell lines of human pancreatic cancer"J Cin.Exp.Cancer Res.. 22. 623-631 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] F.Hata, H.Nishimori, T.Yasoshima et al.: "Profiling Analysis of Differential Gene Expression Between Hematogenous and Peritoneal Metastatic Sublines of Human Pancreatic Cancer Using DNA chip"J Clin.Exp.Cancer Res.. (in press).

    • Related Report
      2003 Annual Research Report
  • [Publications] Nishimori H, Yasoshima T, Hata F, et al.: "A Novel Nude Mouse Model of Liver Metastasis and Peritoneal Dissemination from the Same Human Pancreatic Cancer Line"Pancreas. 24. 242-250 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nomura H, Nishimori H, Yasoshima T, Hata F, et al.: "A new liver metastatic and peritoneal dissemination model established from the same human pancreatic cancer cell line : analysis using cDNA macroarray"Clinical & Experimental Metastasis. 19. 391-399 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nakajima F, Nishimori H, Hata F, et al.: "Gene Expression Screening using cDNA Macroarray for Clarification of the Mechanisms of Peritoneal Dissemination of Pancreatic Cancer"Surgery Today. (in press).

    • Related Report
      2002 Annual Research Report
  • [Publications] Nomura H.: "a new experimental mouse model of peritoneal dissemination of human gastric cancer cells : analysis of the mechanism of peritoneal dissemination using cDNA macroarrays"Jpn. J. Cancer Res.. 92. 748-754 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nishimori H.: "A new peritoneal dissemination model established from human pancreatic cancer cell line"Pancreas. 222. 348-356 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nishimori H.: "a Novel Nude mouse Model of Liver metastasis and Peritoneal Dissemination from the Same Human Pancreatic Cancer Line"Pancreas. (in press).

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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