| Project/Area Number |
13671318
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
HONMA Toshio (2003) HONMA,Toshio, 医学部, 講師 (00253998)
本間 敏男 (2001-2002) 札幌医科大学, 医学部, 助手 (30315494)
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| Co-Investigator(Kenkyū-buntansha) |
KATSURAMAKI Tadashi Sapporo Medical University, School of Medicine, Assistant Professor, 医学部, 助教授 (50253993)
HIRATA Koichi Sapporo Medical University, School of Medicine, Assistant Professor, 医学部, 教授 (50136959)
MUKAIYA Mitsuhiro Sapporo Medical University, School of Medicine, Assistant Professor (00253998)
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| Project Period (FY) |
2001 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2003: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2002: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2001: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | diabetes mellitus / islet transplantation / artificial pancreas |
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| Research Abstract |
We performed homogeneous transplantation using rat islet to find a method for extension of the viable period in islet transplantation. We isolated and purified rat islet from rat pancreas. We can get almost same number of viable islets from rat pancreas and we can make diabetic rats from injecting streptozotocine to rats. First, we tried to make the microcapsule of islet. But, we failed to do it. So, we directly injected islets to portal vein. The data that we got from the method were not stabilized. The period whose blood sugar level was stable was extremely short. We were not able to obtain the result stabilized even if it changed various methods of medication course. It did not clarify about participation of apoptosis in islet transplantation. We performed expression analysis of the Reg protein that is considered to extend a viable period of islets. We inserted cDNA of the Reg protein that was obtained using RT-PCR to expression vectors. The expression efficiency of Reg protein was quite bad. We were not able to get a required quantity of the protein for analysis.
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