Analysis of differential gene expression profiles of gastric cancer cells established from primary tumor and malignant ascites

• Project/Area Number: 13671326
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: SQAKAKURA Chouhei School of Medicine, Assistant Professor, 医学部, 助手 (10285257)
• Co-Investigator(Kenkyū-buntansha): OKAZAKI Yasuhi The Institute of physical and chemical Research, Genomic Sciences center, ゲノム科学総合センター・遺伝子構造・機能研究グループ, チームリーダー (80280733) ; HAGIWARA Akeo School of Medicine, Assistant Professor, 医学部, 助教授 (90198648)
• Project Period (FY): 2001 – 2002
• Project Status: Completed (Fiscal Year 2002)
• Budget Amount: ¥1,500,000 (Direct Cost: ¥1,500,000); Fiscal Year 2002: ¥1,500,000 (Direct Cost: ¥1,500,000)
• Keywords: peritoneal / dissemination / gastric cancer / cDNA microarray

Research Abstract

Differential gene expression profiles of gastric cancer cells established from primary tumor and malignant ascites Abstract Advanced gastric cancer is often accompanied by metastasis to the peritoneum, resulting in high mortality rate. Mechanisms involved in gastric cancer metastasis have not been fully clarified because metastasis involves multiple steps and requires a combination of altered expressions of many different genes. Thus, independent analysis of any single gene would be insufficient to understand all of the aspects of gastric cancer peritoneal dissemination. In this study, we performed a global analysis of the differential gene expression of a gastric cancer cell line established from a primary main tumor (SNU-1) and of other cell lines established from the metastasis to the peritoneal cavity (SNU-5, SNU-16, SNU-620, KATO-III and GT3TKB). The application of a high-density Cdna microarray method made it possible to analyze the expression sequence tags (ESTs). The analysis r evealed the following altered expression such as: (a) up-regulation of CD44 (cell adhesion), keratin 7, 8 and 14 (epitherial marker), aldehyde dehydrogenase (drug metabolism), CD9 and IP3 receptor type3 (signal transduction); (b) down-regulation of IL2 receptor y, IL4-Stat (immune response), p27 (cell cycle) and integrin β4 (adhesion) in gastric cancer cells from malignant ascites. We then analyzed eight gastric cancer cell lines with Northern blot and observed preferential up-regulation and down-regulation of these selected genes in cells prone to peritoneal dissemination. RT-PCR confirmed that several genes selected by DNA microarray were also overexpressed in clinical samples of malignant ascites. It is therefore considered that these genes may be related to the peritoneal dissemination of gastric cancers. The results of this global gene expression analysis of gastric cancer cells with peritoneal dissemination, promise to provide a new insight into the study of human gastric cancer peritoneal dissemination

Research Products

• [Publications] Sakakura C et al.: "Differential gene expression profiles of gastric cancer cells established from primary tumor and malignant as cites"Br.J.Cancer. 87(10). 1153-1161 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Li QL, Ito K, Sakakura C et al.: "Causual relationship between the loss of RUNX3 expression and gastric cancer"Cell. 109. 113-124 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] 阪倉長平, 伊藤嘉明: "RUNX3と胃癌(総説)"細胞工学. 21(10). 1206-1209 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Sakakura C, Hagiwara A, Nakanishi M, Shimomura K, Takagi T, Yasuoka R, Fujita Y, Abe T, Ichikawa Y, Takahashi S, Ishikawa T, Nishizuka I, Morita T, Shimada H, Okazaki Y, Hayashizaki Y, Yamazaki H: "Differential gene expression profiles of gastric cancer cells setablishued from primary tumour and malignant ascites"Br J Cancer. 87(10). 1153-1161 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] *Li QL*, Ito K*, Sakakura C*, Fukamachi H*, Inoue K, Chi XZ, Lee KY, Nomura S, Lee CW, Han SB, Kim HM, Kim WJ, Yamamoto H, Yamashita N, Yano T, Ikeda T, Itohara S, Inazawa J, Abe T, Hagiwara A, Yamagishi H, Ooe A, Kaneda A, Sugimura T, Ushijima T, Bae SC, Ito Y. (*these authors are equally contributed): "Causal Relationship between the Loss of RUNX3"Expression and Gastric Cancer. Cell. 109(1). 113-124 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] *Sakakura C, Hagiwara A, Ikoma H, Hamada T, Taniwaki M, Horiike M, Yamagishi H: "Massive lymphatic fluid leakage during laparoscopic lymphnode biopsy for abdominal malignancy"Hepatogastroenterology. 49(48). 1517-1519 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] *Hagiwara A, Imanishi T, Sakakura C, Otuji E, Kitamura K, Itoi H, Yamagishi H: "Subtotal gastrectomy for cancer located in the greater curvature of the middle stomach with prevention of the left gastric artery"Am J Surg. 183(6). 692-696 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] *Sakura C, Hagiwara A, Kato D, Hamada T, Yamagishi H: "Manifestation of bilateral huge ovarian metastases from colon cencer immediately after the initial operation : report of a case"Surg Today. 32(4). 371-375 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] C.Sakakura et al.: "Differential gene expression profiles of gastric cancer cells established from primary tumor and malignant ascites"Br. J. Cancer. 87(10). 1153-1161 (2002)
• [Publications] Li QL, Ito K, Sakakura C et al.: "Casual relationship between the loss of RUNX3 Expression and gastric cancer"Cell. 109. 113-124 (2002)
• [Publications] 阪倉長平, 伊藤嘉明: "RUNX3と胃癌(総説)"細胞工学. 21(10). 1206-1209 (2002)
• [Publications] Sakakura C et al.: "Polymerase chain reaction for detection of carciroembyonic antigen-expressing tumor cells on milky spots"Int.J.Cancer. 95. 286-289 (2001)
• [Publications] Sakakura C et al.: "Tumor-amplified kinase BTAK is amplified and over expressed in gastric cancers"Brit J Cancers. 84. 824-831 (2001)