| Project/Area Number |
13671332
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Iwate Medical University School of Medicine |
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Principal Investigator |
ISHIDA Kaoru Iwate Medical University School of Medicine, Department of Surgery, Assistant Professor, 医学部, 助教授 (70146041)
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| Co-Investigator(Kenkyū-buntansha) |
MAESAWA Chihaya Iwate Medical University School of Medicine, Department of Pathology, Lecturer, 医学部, 講師 (10326647)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2001: ¥2,600,000 (Direct Cost: ¥2,600,000)
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| Keywords | Biomolecular / Interaction analysis / Bio sensor / esophageal cancer / cancer related genes / 食堂癌 / 浸潤・転移 / MMP / p53 / syclinD1 / EGFR |
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| Research Abstract |
We developed a new system for predicting disease outcome of cancer patients, by using Bio Sensor Chip assay. The assay utilized the surface plasmon resonance technology for the analysis Biomolecular interaction analysis. To overcome the disadvantages of the conventional telomerase assay (TRAP : telomeric repeat amplification protocol) related to PCR artifacts and troublesome post-PCR procedures, we have developed a telomeric repeat elongation (TRE) assay which directly measures telomerase activity as the telomeric elongation rate by biosensor technology using surface plasmon resonance. The assay is rapid and sensitive method, and moreover, allows precise quantitative comparison of a wide range of human cells from somatic cells to carcinoma cells. TRE assay is suitable for practical use in the assessment of telomerase activity in preclinical and clinical trials of telomerase-based therapies, because of its reproducibility, rapidity and simplicity.
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