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Experimental study for reconstruction of portal vein and inferior vena cava in liver surgery

Research Project

Project/Area Number 13671343
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionDokkyo University School of Medicine (2002)
Showa University (2001)

Principal Investigator

YAMAGUCHI Masahiko  Dokkyo University School of Medicine, Professor, 医学部, 教授 (00266149)

Co-Investigator(Kenkyū-buntansha) NAKANO Hiroshi  Showa University, School of Medicine, Assistant Professor, 医学部, 講師 (10241035)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2002: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2001: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordsreconstruction of IVC / hepatectomy / hepatocyte growth factor / ecdothelial seeding
Research Abstract

Results in 2001: Adenovirus vector containing hepatocyte growth factor cDNA (AdHGF) was propagated in 293 cells and concectrated with purification. Vascular endothelial cells (ECs) were isolated from canine jugular vein and cultured in M199+10%FCS. Cytotoxicity and HGF production were investigated to determine the most effective dose of AdHGF. As a result, it was concluded that 50 PFU of AdHGF per cell were the most effective dose.
Results in 2003: Replacement of IVC with PTFE graft where AdHGF-transfected ECs were seeded was performed using 5 dogs after right hepatectomy. Two out of 5 dogs were survived, but three other dogs were dead within a week after the operation. Causes of death included untolerable damages and bleeding by the operation. Survived two dogs were sacrificed 4 weeks after the operation to investigate the liver regeneration and seeds grafts. There was no clot adherent to the graft where viable ECs were attached. Liver regeneration of hepatectomized dogs with seeded IVC graft was not different from hepatectomized dogs with unseeded graft. Serum concentrations of HGF were measured in the operation. However, levels of HGF in all serum samples were less than measurable ones. It is suggested that HGF expression levels from AdHGF-transfected ECs on the graft might not be enough to show biological activity.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Shimabukuro K, Yamaguchi M, et al.: "Hepatocyte growth factor/scatter factor is implicated in the mode"Gynecologic Oncology. 83. 205-215 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M.Yamaguchi, H.Nemoto, et al.: "Effects of Insulin-like growth factor-1 on short bowel syndrome"Eur Serg Res. 33. 291-296 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Okuyama T, Oya M, Yamaguchi Ml: "Budding(sprouting)as a useful prognostic marker in colorectal mucinous carcinoma"Jpn J Clin Oncol. 32. 412-416 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Masahiko Yamaguchi, Genshu Tate, Satoshi Wakasugi, et al.: "Diffuse cystic malformation with early cancer of the stomach"Showa Univ Med Sc. 14. 75-81 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Masahiko Yamaguchi, Genshu Tate, Yasuo Yoshizawa, et al.: "Hepatocellular Carcinoma in a Patient with Acromegaly and High Serum Levels of Insulin-like Growth Factor I : Report of a Case"Surg Today. 32. 1008-1011 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Min Li, Genshu Tate, Masahiko Yamaguchi, et al.: "Hepatocellular Carcinoma in a Patient with Acromegaly and High Serum Levels of Insulin-like Growth Factor I : Report of a Case"Showa Univ J Med Sci. 14. 169-173 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 山口真彦, 熊田馨: "今日の消化器疾患治療指針(第2版):Budd-Chiari症候群"多賀須幸男、三田村圭二、幕内雅敏編集、医学書院. 930 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shimabukuro K, Yamaguchi M, et al.: "Hepatocyte growth factor/scatter is implicated in the mode---"Gynecologic Oncology. 83. 205-215 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M. Yamaguchi, H. Nemoto, et al.: "Effects of Insulin-like growth factor-1 on short bowel syndrome---"Eur Serg Res. 33. 291-296 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Okuyama T, Oya M, Yamaguchi M: "Budding (sprouting) as a useful prognostic marker in colorectal mucinous carcinoma"Jpn Clin Oncol. 32. 412-416 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Masahiko Yamaguchi, Genshu Tate, Satoshi Wakasugi, et al.: "Diffuse cystic malformation with early cancer of the stomach"Showa Univ Med Sc. 14. 75 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Masahiko Yamaguchi, Genshu Tate, Yasuo Yoshizawa, et al.: "Hepatocellular Carcinoma in a Patient with Acromegaly and High Serum Levels of Insulin-like Growth Factor I: Report of a Case"Surg Today. 32. 1008-1011 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Min Li, Genshu Tate, Masahiko Yamaguchi, et al.: "Hepatocellular Carcinoma in a Patient with Acromegaly and High Serum Levels of Insulin-like Growth Factor I: Report of a Case"Showa Univ J Med Sci. 14. 169-173 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Okuyama T, Oya M, Yamaguchi MI: "Budding(sprouting)as a useful prognostic marker in colorectal mucinous carcinoma"Jpn J Clin Oncol. 32. 412-416 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Masahiko Yamaguchi, Genshu Tate, Satoshi Wakasugi, et al.: "Diffuse cystic malformation with early cancer of the stomach"Showa Univ Med Sci. 14. 75-81 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Masahiko Yamaguchi, Genshu Tate, Yasuo Yoshizawa, et al.: "Hepatocellular Carcinoma in a Patient with Acromegaly and High Serum Levels of Insulin-like Growth Factor I : Report of a Case"Surg Today. 32. 1008-1011 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Min Li, Gehshu Tate, Masahiko Yamaguchi, et al.: "A case of chromophobe renal cell carcinoma coincident with malignant gastrointestinal stromal tumor"Showa Univ J Med Sc. 14. 169-173 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 山口真彦, 浅川清人, 高木悦子, 中島政博, 根本洋, 真田裕, 熊田馨: "回盲部合併小腸大量切除に対する実験的検討:成分栄養、IGF-1投与の効果について"外科と代謝・栄養. 36. 49-54 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 山口真彦, 伊津野久紀, 松本匡史, 熊田馨, 盾玄秀, 光谷俊幸: "穿孔性腹膜炎を来した小腸悪性リンパ腫の一例"外科治療. 86. 604-607 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 山口真彦, 熊田馨: "今日の消化器疾患治療指針(第2版):Budd-Chiari症候群"多賀須幸男、三田村圭二、幕内雅敏編集、医学書院. 930 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shimabukuro K, Yamaguchi M, et al: "Hepatocyte growth factor/scatter factor is implicated in the mode ----"Gynecologic Oncology. 83. 205-215 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] M.Yamaguchi, H.Nemoto, et al.: "Effects of Insulin-like growth factor-1 on short bowel syndrome--------------"Eur Serg Res. 33・4. 291-296 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 山口真彦, 吉澤康男, 緑川武正, 他: "重症壊死性膵炎後の膵仮性嚢胞に対する胃瘻を介した外瘻術の1例"外科. 63・1. 106-109 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 山口真彦, 松本匡史, 町田宏, 他: "虫垂粘液嚢胞の術前診断と治療-5症例の検討"消化器外科. 24・7. 1169-1175 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 松本匡史, 山口真彦, 笹屋章示, 他: "十二指腸Gastrointestinal stromal tumor(GIST)の2例"消化器外科. 25・1. 121-127 (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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