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Inducible nitric oxide synthase ameliorates lung injury in rat after gut ischemia-reperfusion

Research Project

Project/Area Number 13671344
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionTokyo Medical University

Principal Investigator

YUKIOKA Tetsuo  Tokyo Medical University, Medicine, Professor, 医学部, 教授 (00182668)

Co-Investigator(Kenkyū-buntansha) 本間 宙  東京医科大学, 医学部, 助手 (60307353)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2003: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2002: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2001: ¥800,000 (Direct Cost: ¥800,000)
KeywordsGut / ischemia-reperfusion / NO / 一酸化窒素 / 虚血再還流障害 / 虚血再灌障害 / NO / ARDS / Gut translocation
Research Abstract

BACKGROUND : The nature of the involvement of an inducible nitric oxide (NO) release in acute conditions has been controversial. We sought to determine whether a relatively selective inducible NO synthase inhibitor, aminooguanidine (AG), ameliorates pulmonary microvascular injury after gut ischemia -reperfusion. METHODS : Anesthetized Wistar rats underwent superior mesenteric artery occlusion for 30 mm and reperfusion for 6 hr (I/R) or sham operation (SHAM). Another set of animals undergoing I/R received an aminoguanidine IP (I/R+AG). Pulmonary vascular permeability was assessed by measuring tissue retention of Evans blue, a dye that binds albumin (EBD). RESULTS : The concentrations of NO_2/NO_3 (lung and plasma) in the I/R group were higher than those of the sham (p<0.01). The lung/plasma EBD ratio In the I/R group was also higher than that of the sham. Treatment with the aminoguanidine prevented this lung injury induced by the gut isehemia-reperfusion. CONCLUSION : Increased lung vascular permeability elicited by gut I/R was significantly attenuated with inhibition of an inducible NO release by AG.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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