| Project/Area Number |
13671351
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Nippon Medical School |
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Principal Investigator |
SUZUKI Seiji Nippon Medical School, School of Medicine, Medical stuff, 医学部, 助手 (60333118)
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| Co-Investigator(Kenkyū-buntansha) |
KAWANAMI Oichi Nippon Medical School, Graduate school of Medicine, Professor, 大学院・医学研究科, 教授 (70096973)
SASAJIMA Koji Nippon Medical School, School of Medicine, Assistant Professor, 医学部, 助教授 (80158930)
EGAMI Kaku Nippon Medical School, School of Medicine, Professor, 医学部, 教授 (60089703)
HASEGAWA Hirokazu Nippon Medical School, School of Medicine, Associate Professor, 医学部, 講師 (60218451)
WATANABE Hidehiro Nippon Medical School, School of Medicine, Associate Professor, 医学部, 講師 (40191788)
カジサデ モハマッド (ガジザデ モハマッド) 日本医科大学, 老人病研究所, 助教授 (30190979)
飯田 信也 日本医科大学, 医学部, 助手 (10267130)
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| Project Period (FY) |
2001 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | CGH / Quantitative real time microsatellite analysis / Microsatellite marker / Stomach cancer / ZNF217 / D8S1801 / D16S3026 / DNA copy number aberration / genetic instability / 定量的real timeマイクロサテライト分析法 / Chromosomal instability / DOP-PCR |
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| Research Abstract |
We detected the relative DNA copy numbers (RCNs) at the target loci in thirty patients with stomach cancer, with quantitative microsatellite analysis (QuMA), Seven microsatellite loci in chromosome 8q (D8S530,D851724,D851801) 16q (D1653140,D1653026) and 20q (D205911,D205185) and one gene specific locus (ZNF217) were selected as the target loci. The RCN was obtained relatively to a pooled reference cocsisting of six microsatellite promer sets selected from the regions where few aberrations have been observed in comparative genomic hybridization (CGH) analysis. Based on the TaqMan PCR system, internal probes used were carring donor (FAM) and acceptor (TAMRA) fluorescent molecules complementary to CA repeat in the microsatellite markers and to one gene specific oligomer in the gene specific marker. Chromosome 8q gain, 20q gain and 16q loss were detected in 18 (60%), 8 (26.7%) and 13 cases (43,3%), respectively. Gains in the RCNs of D8S1801 and D8S1724 were most frequently found (36.7%). There was a significant correlation between the loss of D16S3026 and reduced survival duration (P0.0158), and the simultaneous aberrations of D8S1801 gain and D16S3026 loss (Double marker positive) was significantly associated with reduced survival duration (P=0.0008). According to Cox proportional hazard model, the double marker posive was a significant and independent factor indicationg an unfavorable prognostic factor (RR:17.176,95% Cl:2.782-106.026, P=0.0022). RCN aberrations in tumor tissues determined by QuMA enable to identify the prognostic factors that correlate with clinical outcome of the patients with stomach cancer.
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