| Project/Area Number |
13671352
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Nippon Medical School |
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Principal Investigator |
BO Hideki Nippon Medical School, Center for Digestive Diseases, Assistant Professor, 医学部, 助手 (20333122)
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| Co-Investigator(Kenkyū-buntansha) |
KAWANAMI Oichi Nippon Medical School Institute of Gerontology, Department of Molecular Pathology, Professor, 老人病研究所, 教授 (70096973)
GHAZIZADEH Mohammad Nippon Medical School Institute of Gerontology, Department of Molecular Pathology, Associate Professor, 老人病研究所, 助教授 (30190979)
KURIHARA Yuji Nippon Medical School, Center for Digestive Diseases, Assistant Professor, 医学部, 助手 (80267161)
MORIYAMA Yukichi Nippon Medical School, Center for Digestive Diseases, Professor, 医学部, 教授 (40089701)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2002: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | Esophageal squamous cell carcinoma / Radiation / cDNA microarray / gene expression |
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| Research Abstract |
The mechanism of radiation therapy for esophageal carcinoma is not well understood. To examine the effect of radiation therapy on squamous cell carcinoma at molecular levels, we performed GeneFilters analyses as follows: Three different cell lines comprising of well, moderately, and poorly differentiated squamous cell carcinomas were used. Each cell line was subjected to 2, 4, and 8 Gy radiation. RNA was extracted from each cell line at 0, 15, and 30 min after radiation. Each RNA sample was labeled with 33P and hybridized to GeneFilters (Named Genes; GF-211), filter image was scanned and analyzed using Pathways 3.0 software. Several genes related to cell cycling were down-regulated and several others related to apotosis and matrix synthesis were up-regulated after treatment of the cells with radiation. We also validated some of these genes by a semiquantitative RT-PCR. Overall, we found that radiation at 8 Gy dose gave the best effect at 30 min after radiation. We plan to study the function of these genes in relation to radiation effect in these cell lines.
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