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Investigation of the mechanism of metastasis induction by D4GDI isolated as a metastasis-inducing gene from human colon cancer cell line

Research Project

Project/Area Number 13671354
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionKanazawa Medical University

Principal Investigator

MAEDA Masayo  Kanazawa Medical University, Dept. Pathology, Assistant, 医学部, 助手 (30199632)

Co-Investigator(Kenkyū-buntansha) OTA Takahide  Kanazawa Medical University, Medical Research Institute, Associate professor, 総合医学研究所, 助教授 (10152141)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2001: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordsmetastasis / D4GDI / RhoGDI / ERM proteins / Rac / SW480 colon cancer cell line / 1-1ras1000 / metastasis inducing gene / RhoGDI / D4 / LyGDI / Rho / 転移 / 転移誘導遺伝子
Research Abstract

Rho family GTPases play an important role in a number of processes related to metastasis and RhoGDIs regulate Rho family proteins. We cloned genomic DNA capable of transforming non-metastatic ras-transformed 1-1ras1000 cells into metastatic cells, from colon carcinoma SW480 cells. This DNA contained a truncated human ARHGDIB gene, resulting in a C-terminal truncated form of LyGDI (△C-LyGDI, 166-201 deletion), a member of the RhoGDIs. The stable expression of △C-LyGDI induced pulmonary metastasis in 1-1ras1000 cells, whereas cells expressing full-length LyGDI did not metastasize. △C-LyGDI localized preferentially in the membrane and was detected in a NP-40-insoluble fraction, co-purified with Radixin, Moesin, Rac1, Cdcd42, and RhoA. In △C-LyGDI transfectant an activation state of Rac1 was elevated and △C-LyGDI associated with Rac1-GTP. In accordance with the increase of Rac1-GTP level △C-LyGDI transfectants showed higher invasive ability than mock transfectant. These results suggest that LyGDI functions in the cell membrane to afford spatial regulation of Rho family GTPase signaling through ERM proteins during metastasis.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Ota, T.: "Increased mitotic phosphorylation of histone H3 attributable to AIM-1/Aurora-B overexpression contributes to chromosome number instability"Cancer Res.. 62. 5168-5177 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ota, T.: "Cationic liposomes with plasmid DNA influence cancer metastatic capability"Anticancer Res.. 22. 4049-4052 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ota, T.: "Functional suppression of integrin β4-mediated adhesion caused by in vivo sequential selection for cancer cell intravasation"Anticancer Res.. 21. 205-211 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tatsuka, M.: "Anticarcinogenic effect and enhancement of metastatic potential of BALB/c 3T3 cells by ginsenoside Rh2"Jpn. J. Cancer Res.. 92. 1184-1189 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ota, T.: "Functional suppression of integrin β4-mediated adhesion caused by in vivo sequential selection for cancer cell intravasation"Anticancer Res.. 21(1A). 205-211 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tatsuka, M.: "Anticarcinogenic effect and enhancement of metastatic potential of BALB/c3T3 cells by ginsenoside Rh_2"Jpn. J. Cancer Res.. 92(11). 1184-1189 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ota, T.: "Cationic liposomes with plasmid DNA influence cancer metastatic capability"Anticancer Res.. 22(6C). 4049-4052 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ota, T.: "Increased mitotic phosphorylation of histone H3 attributable to AIM-1/Aurora-B overexpression contributes to chromosome number instability"Cancer Res.. 62(18). 5168-5177 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ota, T.: "Increased mitotic phosphorylation of histone H3 attributable to AIM-1/Aurora-B overexpression contributes to chromosome number instability"Cancer Res.. 62・18. 5168-5177 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ota, T.: "Cationic liposomes with plasmid DNA influence cancer metastatic capability"Anticancer Res.. 22・6C. 4049-4052 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ota, T.: "Functional suppression of integrin β 4-mediated adhesion caused by in vivo sequential selection for cancer cell intravasation"Anticancer Res.. 21・1A. 205-211 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tatsuka, M.: "Anticarcinogenic effect and enhancement of metastatic potential of BALB/c 3T3 cells by ginsenoside Rh2"Jpn. J. Cancer Res.. 92・11. 1184-1189 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ota, T.: "Functional suppression of integrin β4-mediated adhesion caused by in vivo sequential selection for cancer cell intravasation"Anticancer Research. 21・1A. 205-211 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tatsuka, M.: "Anticarcinogenic effect and enhancement of metastatic potential of BALB/c 3T3 cells by ginsenoside Rh2"Jpn. J. Cancer Res.. 92・11. 1184-1189 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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