Detection of bronchial squamous dysplasia using high magnification bronchovideoscopy and angiogenesis of multi step carcinogenesis of squamous cell carcinoma
| Project/Area Number |
13671376
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | Chiba University |
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Principal Investigator |
SHIBUYA Kiyoshi Chiba University, University Hospital, Lecturer, 医学部附属病院, 講師 (20302565)
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| Co-Investigator(Kenkyū-buntansha) |
SEKINE Yasuo Chiba University, Graduate School of Medicine, Assistant, 大学院・医学研究院, 助手 (70312957)
IIZASA Toshihiko Chiba University, Graduate School of Medicine, Associate Professor, 大学院・医学研究院, 助教授 (10272303)
FUJISAWA Takehiko Chiba University, Graduate School of Medicine, Professor, 大学院・医学研究院, 教授 (80110328)
斎藤 幸雄 千葉大学, 大学院・医学研究院, 講師 (60261905)
馬場 雅行 千葉大学, 大学院・医学研究院, 助教授 (00143305)
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| Project Period (FY) |
2001 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2003: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 2002: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2001: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | high-magniftcationbronchovideoscopy / bronchial dysplasia / multi step carcinogenesis / neovascularization |
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| Research Abstract |
The purpose of this study was to investigate the value of high-magnification bronchovideoscopy in detailed examination of dysplasia in the bronchial mucosa of patients exhibiting abnormal mucosal fluorescence. A high-magnification bronchovideoscope (XBF 200HM2) was used in this study. A total of 31 patients with sputum cytology suspicious or positive for malignancy were entered into the study. Conventional white-light examination was first performed on patients under local anesthesia. Fluorescence bronchoscopic examination was also caned out using a LIFE lung-system. Then, high-magnification observation was performed by high-magnification bronchovideoscopy primarily at sites of normal and abnormal fluorescence to examine the microvascular network in the bronchial mucosa. Images obtained by high-magnification bronchovideoscopy were compared with pathological diagnoses from bronchial biopsy specimens. For the vascular patterns obtained by high-magnification bronchovideoscopy, vascular ar
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ea ratios were calculated using an image-analyzing apparatus and compared. Mascular networks with regular patterns were observed in 20 of 22 abnormal fluorescence sites with bronchitis. However, vascular networks with increased vessel growth and complex networks of tortuous vessels of various sizes were observed in 15 of 21 abnormal fluorescence sites with dysplasia. There was a significant difference between bronchitis and dysplasia (OR-25, 95%CI, 5.5 to 113, p<0.0001). Vascular area ratios with the means (95% confidence interval [CI])from the 16 normal bronchial epithelium at sites of normal fluorescence, 22 bronchitis and 21 dysplasia at sites of abnormal fluorescence, were 0.054 (95%CI=0.039 to 0.07), 0.095 (95%CI=0.072 to 0.118), and 0.173 (95%CI=0.143 to 0.203), respectively. The results indicated a statistically significant increase in vascular area values in the three group (p <0.0001). Our results showed that areas of increased vessel growth and complex networks of tortuous vessels in the bronchial mucosa detected by high magnification bronchovideoscope at sites of abnormal fluorescence may allow for discrimination between bronchitis and dysplasia Less
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Report
(4 results)
Research Products
(14 results)
