| Project/Area Number |
13671381
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
HASEGAWA Seiki Graduate School of Medicine, assistanl professor, 医学研究科, 助教授 (10252438)
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| Co-Investigator(Kenkyū-buntansha) |
WADA Hiromi Graduate School of Medicine, Professor, 医学研究科, 教授 (90167205)
HYU HYON Suong Institwte for Frotier Medicine Sciences, asistant professor, 再生医科学研究所, 助教授 (90283655)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2001: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | green tea polyphenol / lung preservation / frachea preservation / ischemial reperfusior jnjury / physical characteristic evaluatim / epgallocatechin gollcte / ポリフェノール / 気管 / 保存液 / 物理的特性 / EK-K / 気管移植 / エピガロカテキンガレード / 臓器保存 / 気管腫瘍 / 気管狭容 |
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| Research Abstract |
We have examined the effect of Green Tea Polyphenol (GPT) for the preservation of trachea and lung. 1. Inhibition of cytokine production; We proved that GPT blocks oxygen-induced IL-8 production and mRNA expression in human epithelial cells with inhibition of JNK and p38, and p44/42. 2. The effect for Ischemia/Reperfusion (I/R) injury after cold preservation; We examined the effect of GPT as a preservation solution by adding various doses of GPT to our preservation solution (ET-K) with an ex vivo rat lung perfusion model. As a result, GPT did not show a preservative effect after cold preservation of rat lung. 3. The effect for Ischemia/Reperfusion (I/R) injury after warm preservation; We examined the effect of GPT against warm I/R injury with an ex vivo rat reperfusion model. As a result, GPT did not relieve the I/R injury after warm preservation of rat lung. 4. Physical characteristic evaluation of rat trachea; We stretched the isolated rat tracheas after cold preservation and evaluated the viscoelasticity of them by Stress-Strain curve obtained by Quasi-static measurement. Each trachea was preserved for a fixed period of time (24hr and 7days) with a preservation solution adding GPT or not. After 24hr preservation, there are no differences among each group. After 7days cold preservation, all tracheas in a GPT adding group became significantly rigid. GPT was not an effective component as a preservation solution of trachea.
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