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TISSUE ENGINEERING CARDIAC CHORDAE

Research Project

Project/Area Number 13671410
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Thoracic surgery
Research InstitutionTOKYO WOMEN'S MEDICAL UNIVERSITY

Principal Investigator

KOSAKA Yoshimichi  TOKYO WOMEN'S MEDICAL UNIVERSITY, Assistant Professor, 医学部, 助手 (90297507)

Co-Investigator(Kenkyū-buntansha) SHINOKA Toshiharu  TOKYO WOMEN'S MEDICAL UNIVERSITY, Associate Professor, 医学部, 講師 (20192122)
AOKI Mitsuru  TOKYO WOMEN'S MEDICAL UNIVERSITY, Associate Professor, 医学部, 講師 (80175736)
KUROSAWA Hiromi  TOKYO WOMEN'S MEDICAL UNIVERSITY, Professor, 医学部, 教授 (50075511)
松村 剛毅  東京女子医科大学, 医学部, 助手 (20297469)
渡辺 学  東京女子医科大学, 医学部, 助手 (10297468)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2001: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsTISSUE ENGINEERING / CARDIAC CHORDAE / AUTOLOGOUS CELLS / ENDOTHELIAL CELLS / MITRAL VALVE / CHORDAL REPLACEMENT / 再生医工学 / 人工腱索 / 細胞培養 / 引張試験
Research Abstract

Various vascular grafts are commonly used in the reconstruction of cardiovascular tissues. However, currently used prosthetic or bioprosthetic materials lack growth potential and, therefore, subsequently require replacement in pediatric patients as they mature. Tissue engineering (TE) is a new discipline that offers the potential to create replacement structures from autologous cells and biodegradable polymer scaffolds. Because TE constructs contain living cells, they may have the potential to grow, self-repair, and self-remodel. Tissue Engineered Autografts (TEAs) were made by seeding 4-6 x 10^6 of mixed cells obtained from femoral veins of mongrel dogs onto valved patch-shape biodegradable polymer scaffolds composed of a polyglycolic acid (PGA) non-woven fabric sheet and a co-polymer of l-lactide and-caprolactone (N=4). After 7 days, the tricuspid chordae of the same dogs were replaced with TEAs. After 3, 4, 5 and 6 months, angiographies were performed, and the dogs were sacrificed. … More The implanted TAs were examined both grossly and immunohistologically. The implanted TEAs showed no evidence of stenosis or dilatation. No thrombus was found inside the TEAs, even without any anticoagulation therapy. Remnants of the polymer scaffolds were not observed in all specimens, and the overall gross appearance appeared similar to that of native pulmonary valves. Immunohistological staining revealed the presence of Factor VIII positive nucleated cells at the luminal surface of the TEAs. In addition, lesions were observed where α-smooth muscle actin and desmin positive cells existed. Implanted TEAs contained a sufficient amount of extracellular matrix, and showed neither occlusion nor aneurysmal formation. In addition, endothelial cells were found to line the luminal surface of each TEA. These results strongly suggest that ideal' grafts with anti-thrombogenicity can be produced. Because both our laboratory and clinical experiences are quite encouraging, we suggest that the TE approach may play an important role as an alternative method to transplantation and to the use of artificial organs in the field of pediatric cardiovascular surgery. Less

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] T.Shinoka: "Tissue Engineered Heart Valves : Autologous Cell Seeding on Biodegradable Polymer Scaffold"Artificial Organ. 26. 522-526 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Naito Y, Imai Y, Shin oka T, Aoki M, Kashiwagi, Konuma T, Kurosawa H.: "A Successful Clinical Application of Tissue Engineered Graft for Extracardiac Fontan Operation"Journal of Thoracic & Cardiovascular Surgery. 125. 419-420 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Hiramatsu, Y.Imai, H.Kurosawa, Y.Takanashi, M.Aoki, T.Shin'oka: "Effects of dilutional and modified ultrafiltration in plasma endothelin-1 and pulmonary vascular resistance after the fontan procedure"Ann Thorac Surg. 73. 862-865 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kashiwagi J, Imai Y, Aoki M, Shin'oka T, Hagino I, Nakazawa M: "An arterial switch operation for a concordant crisscross heart with the complete transposition of the great arteries"Journal of Thoracic & Cardiovascular Surgery. 124. 176-178 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Hiramatsu, Y.Imai, H.Kurosawa, Y.Takanashi, M.Aoki, T.Shin'oka: "Midterm results of surgical treatment of systemic ventricular outflow obstruction in Fontan patients"Ann Thorac Surg. 73. 1282-1285 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shin'oka T: "Tissue Engineered Heart Valves : Autologous Cell Seeding on Biodegradable Polymer Scaffold"Artificial Organ. 26. 522-526 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shinoka T, Imai Y, Ikada Y: "Transplantation of a Tissue Engineered Pulmonary Artery"The New England Journal of Medicine. 344. 532-533 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Naito Y, Imai Y, Shin'oka T, Kashiwagi J, Aoki M, Watanabe M, Matsumura G, Kosaka Y, Konuma T, Hibino N, Murata A, Miyake T, Kurosawa HA: "Successful Clinical Application of Tissue Engineered Graft for Extracardiac Fontan Operation"J Thorac Cardiovasc Surg. 125. 419-420 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kashiwagi J, Imai Y, Aoki M, Shin'oka T, Hagino I, Nakazawa M: "An arterial switch operation for a concordant crisscross heart with the complete transposition of the great arteries"J Thorac Cardiovasc Surg. 124. 176-178 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Watanabe M, Shin'oka T, Tohyama S, Hibino N, Konuma T, Matsumura G, Kosaka Y, Ishida T, Imai Y, Yamakawa M, Ikada Y, Morita S: "Tissue engineered vascular autograft -inferior vena cava replacement in a dog model -"Tissue Engineering. 7. 429-439 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yasuharu Imai, Kazuhiro Seo, Mitsuru Aoki, Toshiharu Shin'oka, Takeshi Hiramatsu, Jun Ohta: "Double-Switch Operation for Congenitally Corrected Transposition"Seminar in Thoracic and Cardiovascular Surgery Pediatric Cardiac Surgery Annual 2001. 4. 16-33 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hibino N, Imai Y, Aoki M, Shinoka T, Hiramatsu T: "Double Switch Operation for Superior-Inferior Ventricles"Annals of Thoracic Surgery. 72. 2119-2121 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Shinoka: "Tissue Engineered Heart Valves : Autologous Cell Seeding on Biodegradable Polymer Scaffold"Artificial Organ. 26. 522-526 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Naito Y, Imai Y, Shin'oka T, Aoki M, Kashiwagi, Konuma T, Kurosawa H: "A Successful Clinical Application of Tissue Engineered Graft for Extracardiac Fontan Operation"Journal of Thoracic & Cardiovascular Surgery. 125. 419-420 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Hiramatsu, Y.Imai, H.Kurosawa, Y.Takanashi, M.Aoki, T.Shin'oka: "Effects of dilutional and modified ultrafiltration in plasma endothelin-1 and pulmonary vascular resistance after the fontan procedure"Ann Thorac Surg. 73. 862-865 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kashiwagi J, Imai Y, Aoki M, Shin'oka T, Hagino I, Nakazawa M: "An arterial switch operation for a concordant crisscross heart with the complete transposition of the great arteries"Journal of Thoracic & Cardiovascular Surgery. 124. 176-178 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Hiramatsu, Y.Imai, H.Kurosawa, Y.Takanashi, M.Aoki, T.Shin'oka: "Midterm results of surgical treatment of systemic ventricular outflow obstruction in Fontan patients"Ann Thorac Surg. 73. 1282-1285 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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