The role of telomere in regulating the brain tumor proliferation
| Project/Area Number |
13671431
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
NAKASU Satoshi Shigauniversity of Medical Science, Neurosurgery, Associate professor, 医学部, 助教授 (00135477)
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| Co-Investigator(Kenkyū-buntansha) |
KANEKO Masaharu Shiga University of Medical Science, Neurosurgery, Assistant, 医学部, 助手 (50303772)
中島 正之 滋賀医科大学, 医学部, 助手 (40283569)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2001: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | meningioma / p21^<cip1 / waf-1> / p27^<kip-1> / p16 / telomere / telomerase / MIB-1 / hTERT / meninqioma / tolomerase / uTERT / p27 / p21 |
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| Research Abstract |
We investigated the relationship between proliferation index, expression of cell cycle inhibitors and telomerase activity in brain tumors. In meningiomas, telomerase activity was found only in high grade tumors. We found correlation between the expression of hTERT and proliferating index (MIB-1). However, immunohistochemical study revealed that even in benign tumor hTERT was expressed in several cells that were MIB-1 positive. We demonstrated that these hTERT positive cells were definitely meningioma cells by double staining with EMA and hTERT. Tumors with high rate of hTERT positive cells are usually atypical or anaplastic meningiomas and exhibited a high recurrence rate. Similar findings were also found in gliomas.
Expression of p27 was frequently observed in meningiomas. The relationship between the proliferation indices and frequency of p27 expression was not distinct. However, we found correlation between cellularity and p27 expression . p27 was also expressed in perinecrotic area. Expression of p21 was relatively rare and correlated with the proliferation index. On the contrary,expression of p16 adversely correlated with proliferation index. Calcified tumors often showed p16 positive cells, and they usually lack hTERT expression. However, we could not demonstrated the correlation between p16 expression and telomere length.
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Report
(3 results)
Research Products
(8 results)
