| Project/Area Number |
13671466
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | JUNTENDO UNIVERSITY |
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Principal Investigator |
ARAI Hajime JUNTENDO UNIVERSITY School of Medicine, professor, 医学部, 教授 (70167229)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAJIMA Masakazu JUNTENDO UNIVERSITY School of Medicine, lecturer, 医学部, 講師 (60200177)
OKUDA Osamu JUNTENDO UNIVERSITY School of Medicine, lecturer, 医学部, 講師 (30265996)
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| Project Period (FY) |
2001 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 2003: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2002: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | Amniotic epitherial cell / ischemic brain / transplantation / 神経細胞 / 神経再生 / 神経幹細胞 |
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| Research Abstract |
We investigated the potential use of rat amniotic epitherial cells as donor cells for transplantation-based therapy in brain ischemia.
Methods are listed as below; primary culture of amniothic epitherial cells; immunocytochmical staining of amniotic epitherial cells; execution of ischemic brain(infarction)model rat; genic induction of GFP into the amniotic epitherial cells; and transplantation of amniotic epitherial cells into the model rat brain.
Results: The amniotic epitherial cells are collected abundantly in E17 rats and the most lasting. On immunocytochmical staining, the rat amniotic epitherial cells were positive reaction to neuronal markers, micro-tubule-associated protein 2, neurofilament and nestin. The induction of retroviral vector enhanced by green fluorescent protein(GFP) succeeded. The grafted amniotic epitherial cells revealed the migration into the brain(CA1 pyramidal layer) and the existence of that cells was confirmed.
Conclusion: these results suggest that intracerebral transplantation of amniotic epitherial cells may have therapeutic potential for the treatment of ischemic damage in neuronal disorders.
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