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Studies on the invasive potential of malignant gliomas

Research Project

Project/Area Number 13671469
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionTeikyo University

Principal Investigator

NAGASHIMA Tadashi  Teikyo University, Shool of Medicine, Professor (70217991)

Co-Investigator(Kenkyū-buntansha) MATSUNO Akira  Teikyo University, School of Medicine, Associate Professor (00242058)
TANAKA Hideki  Teikyo University, School of Medicine, Staff Assistant (50276713)
KATAYAMA Haruko  Teikyo University, School of Medicine, Staff Assistant (40307187)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywordsinvasion / fast blue / HGF / MIB-1SI / P53 / GEAP / fibronectin / グリオーマ / 多発性腫瘍 / 弧発性腫瘍 / MIB-1 / ファイブロネクチン / ラミニン / ヌードラット / 蛍光染色 / トグリオーマ株細胞 / 腫瘍細胞浸潤 / U251MG / U87MG
Research Abstract

5×10^5U87MG cells stained with fast blue were inoculated in to the right basal ganglia of nude rat (F344/Njcl-mu). Five and 10 days after inoculation, hepatocytic growth factor (HGF) was injected into the brain of each nude rat. Two to three weeks after tumor inplantation, the brain of each nude rat were excised, fixed, and cut into 6 micro mm frozen sections.We observed the brain tumor and its surrounding brain tssue of each frozen section with fluorescein microscope. We found that tumor cells were invaded into the sorrounding brain tissue markedly as compared with control section which prepared from the nude rat without HGF administration. This result suggests that HGF increase the invasive potential of glial tumor cells.
We investigated the biological characteristics such as proliferative potential estimated from MIB-1 SI, and expression of P53 GFAP, fibronectin of multicentric glioblastomas, and compare them with those of singlecentric glioblastomas. In this study, paraffin enbedded sections of four multicentric glioblastomas and five singlecentric glioblastomas were used for immunohistochemical staining of MIB-1, P53, GEAP and fibronectin. The value of MIB-1SI and the degree of expression of P53, GFAP, fibronectin appere to be no significant difference between multicentric glioblastomas and singlecentric glioblastomas. These results suggest that some other intrinsic factors, such as MMP-2 and VEGF, may participate in invasion of glioblastomas.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (19 results)

All 2002 2001 Other

All Journal Article (9 results) Book (1 results) Publications (9 results)

  • [Journal Article] 神経膠腫の成長因子2002

    • Author(s)
      長島正
    • Journal Title

      脳神経外科Advanced Practice 5

      Pages: 164-167

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] 電顕in situ hybridization(ISH)による分泌蛋白とそのmRNAの細胞内局在2002

    • Author(s)
      松野彰, 他
    • Journal Title

      日本電子顕微鏡学会誌 37

      Pages: 85-88

    • NAID

      10009532644

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Growth factor of gliomas2002

    • Author(s)
      Nagashima T
    • Journal Title

      Neurosurgery Advanced Practice (Takakura K, et al ed) (Medical View) 5

      Pages: 164-167

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Intracellular localiztion of secreted proteins and their mRNAs, detected by ultrastractural in situ hybridization method2002

    • Author(s)
      Matsuno A, Nagashima T, Takekoshi S, Utunomiya H, Oosugi Y, Osamura Y
    • Journal Title

      J of Japan Electromicroscopic Sociaty 37

      Pages: 85-88

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] 腫瘍マーカー2001

    • Author(s)
      長島正
    • Journal Title

      Clinical : Neuroscience 19

      Pages: 307-309

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Drug-resistance gene exppression and progression of astrocytic tumor2001

    • Author(s)
      Tanaka S, 他
    • Journal Title

      Brain Tumor Pathology 18

      Pages: 131-137

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Tumor marker2001

    • Author(s)
      Nagashima T
    • Journal Title

      Clinical Neuroscience 19-3

      Pages: 307-309

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Confocal Laser Scaning Microscopy2001

    • Author(s)
      Matsuno A, Itoh J, Nagashima T, Osamura Y, Watanabe K
    • Journal Title

      Morphology Metuods Cell and Molecular Biology Techniques (Lloyd RV ed) (Human Press) 10

      Pages: 165-180

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Drug-registance gene expression and progression of astrocytic tumors2001

    • Author(s)
      Tanaka S, Kobayashi I, Oka H, Fujii K, Watanabe T, Nagashima T, Hori T
    • Journal Title

      Brain Tumor Pathol 18-2

      Pages: 131-137

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Book] Morphology Method: Cell and Molecular Biology Techniques2001

    • Author(s)
      Matsuno A, 他
    • Total Pages
      422
    • Publisher
      Humana Press
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 長島 正: "神経膠腫の成長因子"脳神経外科Advance Practice. 5. 164-167 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 松野 彰: "ラット下垂体GHRHとSRIFの投与下でのRab3b蛋白の形態学的名発現様式とGH分泌との関連"ホルモンと臨床O2臨床増刊号内分泌病理学最近の進歩2002. 50. 41-44 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 長島 正: "内科学"杉本恒明 他 総集編 朝倉書店. 2110 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 長島 正 他: "中枢神経原発悪性リンパ腫"Annual Review神経. 2001. 186-194 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Matsuno A: "Prolactin-secreting gangliocytoma:Letter to the editor"J Neurosurg. 95. 167-168 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Matsuno A: "Endocrinologic aspects of twenty-three patients with Rathke's cleft cyst"Endocrinologist. 11. 245-246 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 松野彰: "empty sellaを合併し末端肥大症を呈した蝶形骨洞内異所性下垂体腺腫の1例"ホルモンと臨床49巻'01夏季増刊号内分泌病理学最近の進歩. 49. 84-89 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Matsuno A: "PROTOCOLS 8 Ultrastructural in situ hybridization"Humana Press Chapter. 8. 129-144 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 長島 正(分担): "脳神経外科 Advanced Practice 5神経膠腫"高倉公朋 メジカルビュー社. 190 (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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