Study of newly autologous chondrocyte implantation using by gene transfer of hyaluronan synthase
| Project/Area Number |
13671491
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Chiba University |
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Principal Investigator |
WADA Yuichi Chiba University, University Hospital, Assistant, 医学部附属病院, 助手 (10282485)
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| Co-Investigator(Kenkyū-buntansha) |
TAKASE Kan Chiba University, Graduate School of Medicine, Assistant, 大学院・医学研究院, 助手 (40333489)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2001: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | hyaluronan / gene transfer / chondrocyte implantation / ATDC5 / retrovirus vector / ヒアルロン酸合成酵素 |
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| Research Abstract |
In the present study, plasmids containg HAS2 under the PMX-IRES-GFP retrovirus vector were transfected into undifferentiated ATDC5. As a control, cells transfected with the empty vector were used. ATDC5 cells differentiated in the presence of insulin and RT-PCR was performed to detect HAS2 m RNA expression and we evaluated visualization of pericellular coats which were composed of newly synthesized HA using by immunofluorescent staining and analyzed HA concentration in culture medium by high performance liquid chromatography. The signal of the cells transfected with HAS2 was visually much stronger than that of control cells. The HA concentration of the cells transfected with HAS2 in culture medium was much higher than that of control throughout 21 days. By transfection of HAS2 with retrovirus, we are able to expect the continuous synthesis of HA. Therefore this technique may be a great significance in autologous chondrocyte implantation.
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Report
(3 results)
Research Products
(7 results)
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[Publications] Kan Tsuchiya, Yuichi Wada, Kan Takase, Kouichi Nakagawa, Takeshi Yamashita, Hiroki Sakai, Norimasa Takahashi, Atsuya Watanabe, Rie Koh, Yoshikuni Kawaguchi and Hideshige Moriya: "EVALUATION OF HYALURONAN SYNTHESIS BY RETROVIRUS-MEDIATED GENE TRANSFER OF HYALURONAN 2"InternationaI Society of Arthroscopy, Knee Surgery and Orthopaedic Sports Medicine Congress Abstracts. 5. 144 (2003)
Description
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