| Project/Area Number |
13671511
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
ISHIDA Osamu Hiroshima University, Graduate School of Biomedical Science, Assistant, 大学院・医歯薬学総合研究科, 助手 (00243551)
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| Co-Investigator(Kenkyū-buntansha) |
SUNAGAWA Toru Hiroshima University, University Medical Hospital, Assistant, 医学部付属病院, 助手 (40335675)
YASUNAGA Yuji Hiroshima University, University Medical Hospital, Assistant Professor, 医学部付属病院, 講師 (40253075)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2002: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | FGF-2 / osteonecrosis / angiogenesis / treatment / growth factor |
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| Research Abstract |
The aim of this study is to accelerate the angiogenesis in necrotic bone by combining vascular bundle implantation and FGF-2 administration
Twenty-four Japanese white rabbits were used. A portion of rabbit iliac crest bone was removed as a free bone graft and frozen in liquid nitrogen to ensure complete cellular necrosis. A narrow hole was created in the bone and the graft was placed in the proximal thigh. In group 1, FGF-2 was injected into the hole at a single dose of 100 micrograms, and the saphenous artery and its venae comitantes were passed through the hole of the bone. In group 2, injection of saline into the hole and vascular bundle implantation was used as a control. Neovascularization around the vessel was evaluated at weeks 1 and 2 after vestment
Neovascularization was observed along the implanted vascular bundle. The vessel density gradually increased from week 1 to week 2 in both groups. At 1 week after surgery, the vessel density in group 1 (0.148) was significantly higher than in group 2 (0.050). At 2 weeks after surgery, the mean of the vessel density in group 1 was 0.375 and that in group 2 was 0. 194. This difference was also statistically significant. The length of vessels at 1 week after surgery in group 1 (0.862mm) was significantly higher than in group 2 (0.330mm). At 2 weeks after surgery, the mean of the vessel length in group 1 was 2.625mm and that in group 2 was 1.383mm. This difference was also statistically significant
A local single injection of FGF-2 improved surgical angiogenesis in necrotic bone in this study. As FGF-2 is recognized as a potent mitogen for a variety of mesenchymal cells, the combination of vascular bundle implantation and FGF-2 administration may contribute to the treatment of avascular necrosis of bone
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