| Project/Area Number |
13671544
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Tokyo Women's Medical University |
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Principal Investigator |
KIM Kang-jung Tokyo Women's Medical University, Department of Orthopaedics, associate professor, 医学部, 講師 (50231288)
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| Co-Investigator(Kenkyū-buntansha) |
IWASE Miho Tokyo Women's Medical University, Department of Orthopaedics, assistant professor, 医学部, 助手
KOTAKE Shigeru Tokyo Women's Medical University, Department of Orthopaedics, assistant professor, 医学部, 講師 (00234774)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2002: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2001: ¥600,000 (Direct Cost: ¥600,000)
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| Keywords | arthroplasty / osteolysis / wear debris / osteoclast / osteoprotegerin |
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| Research Abstract |
A bone cement prosthesis was inserted into the femur of thirty rats and divided into three groups (negative control, positive control, and OPG group). After peri-prosthetic osteolysis was established in four weeks, OPG group were intravenously injected with 10mg/kg of OPG every week until they were sacrificed at 8 weeks. Peri-prosthetic bone resorption was evaluated with bone mineral density, and histomorphometric analysis was performed by measuring the area of interface membrane as well as grading the membrane according to the inflammatory characteristics. Radiographs were evaluated for the presence of osteolysis with a blind manner.
Results : The results of DEXA showed that peri-prosthetic bone resorption was significantly suppressed in OPG group compared to the other groups (p<0.05) (negative control group ; 225, positive control group ; 218, OPG group ; 233mg/cm^2, respectively). Histomorphometric analyzes showed the less area as well as less inflammatory grading of the interface membrane in OPG group compared to other groups (p<0.05). Radiographic osteolysis was seen less frequently in OPG group compared to other groups (p<0.05) (1/8, 7/10, 3/10, respectively).
Discussion : The present study demonstrates that OPG has significantly suppressed or even restored peri-prosthetic osteolysis in our animal model. OPG may be a possible agent to retain or restore the bone stock before revision surgery for failed prostheses.
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