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Proliferative potential of frozen chodrocytes and their transplantation for the treatment of osteoarthritis.

Research Project

Project/Area Number 13671545
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionNihon University

Principal Investigator

RYU Junnosuke  Nihon Univ., School of Medicine, Professor, 医学部, 教授 (30096854)

Co-Investigator(Kenkyū-buntansha) ISHII Takao  Nihon Univ., School of Medicine, Assistant, 医学部, 助手 (40277447)
SAIGO Kaichiro  Nihon Univ., School of Medicine, Assistant, 医学部, 助手 (00339318)
SAKAMOTO Masamitsu  Nihon Univ., School of Medicine, Assistant, 医学部, 助手 (40409016)
MORI Sei  Nihon Univ., School of Medicine, Assistant, 医学部, 助手 (80386028)
KUDOH Taro  Nihon Univ., School of Medicine, Assistant, 医学部, 助手 (50386041)
福島 一雅  日本大学, 医学部, 講師 (40328737)
相部 和士  日本大学, 医学部, 助手 (60318392)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2001: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordschondrocyte / proteoglican / TGF-β / bFGF / IL-1α / growth factor / hyaluronic acid / cryopreservation / 凍結軟骨細胞 / TGF-β / 軟骨組織 / 豚 / chondrocyte / proteoglycan / bFGF / growth factor / collagen / Crypeservation / Cryopreservation
Research Abstract

Preclinical experiments on frozen chondrocytes derived from articular cartilage were performed for their transplantation for repair of articular cartilage defects in osteoarthritis.
Experiment 1. Animal chondrocytes
Chondrocytes obtained from young female swine were isolated and kept frozen at -196℃ for various periods of time. Effects of the length of cryopreservation on the rate of viable cells and their ability to synthesize proteoglican (PG) and collagen were studied. Effects of various growth factors were assessed by keeping the cells frozen for one of them. The results are as follows :
1) The rates of viable cells in the cells kept frozen for 2 weeks or less and further up to 12 weeks were 90% and 80%, respectively.
2) The frozen chondrocytes were not significantly different from unfrozen chondrocytes in ability to synthesize PG and collagen, regardless of the duration of freezing, whether they were kept frozen for 2, 4, 8 or 12 weeks.
3) Growth factors such as TGF-β, IGF-1 and bFGF invariably increased the abilities to synthesize PG and collagen even in cells kept frozen for as long as 12 weeks.
Experiment 2. Human chondrocytes
After patient's agreement, cartilages were collected from femoral heads excised during operations for femoral neck fractures. The results are as follows :
1) The rates of viable cells were 82.7%, 80.7% and 78.2% in the cells kept frozen for 2 or less, 4 and 8 weeks, respectively.
2) The abilities to synthesize PG and collagen showed no significant change even in cells kept frozen for further up to 8 weeks.
3) Growth factors (TGF-β, IGF-1 and bFGF) increased the abilities to synthesize PG and collagen. The increased abilities remained unchanged even in the cells kept frozen for 8 weeks.
In brief, the chondrocytes kept frozen for as long as 8 to 12 weeks were considered transplantable.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (17 results)

All 2005 2003 2002 2001 Other

All Journal Article (12 results) Publications (5 results)

  • [Journal Article] 実験的OA軟骨細胞におけるヒアルロン酸ナトリウムの効果2005

    • Author(s)
      石井隆雄, 龍順之助, 斎藤修, 清水一郎, 関雅之, 工藤太郎
    • Journal Title

      関節外科 24(1)

      Pages: 37-43

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Effects of hyaluronic acid on experimentally induced osteoathritic chondrocytes.2005

    • Author(s)
      Ishii T, Ryu J, Saito S, Shimizu I, Seki M, Kudoh T
    • Journal Title

      J of Joint Surg 24(1)

      Pages: 37-43

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] 軟骨におけるIL-1による軟骨破壊に対する抑制作用2003

    • Author(s)
      工藤太郎, 龍順之助, 斎藤修, 石井隆雄, 関雅之, 森聖, 坂本雅光
    • Journal Title

      日本整形外科学会誌 77(8)

      Pages: 1136-1136

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Response of cryopreserved chondrocytes to growth factors.2003

    • Author(s)
      Sakamoto M, Ryu J, Kudoh T, Mori S
    • Journal Title

      The Nihon University Journal of Medicine 45(2)

      Pages: 65-62

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] 変形性膝関節症に対するヒアルロン酸注入療法2003

    • Author(s)
      龍順之助
    • Journal Title

      臨床リウマチ 別冊 15

      Pages: 57-62

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Effect of inhibition of IL-1 stimulated pig cartilage degradation.2003

    • Author(s)
      Kudoh T, Ryu J, Saito S, Ishii T, Seki M, Mori S, Sakamoto M
    • Journal Title

      J. Jpn. Orthop. Assoc. 77(8)

      Pages: 1136-1136

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Response of cryopreserved chondrocytes to growth factors.2003

    • Author(s)
      Sakamoto M, Ryu J, Kudoh T, Mori S
    • Journal Title

      Nihon Univ. J. Med. 45(2)

      Pages: 65-76

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Intraarticular sodium hyaluronate in treatment of patients with osteoarthritis of the knee.2003

    • Author(s)
      Ryu J
    • Journal Title

      Clin Rheumatol 15

      Pages: 57-62

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] 凍結軟骨細胞におけるヒアルロン酸と成長因子の影響2002

    • Author(s)
      坂本雅光, 龍順之助, 石井隆雄, 工藤太郎, 森聖
    • Journal Title

      日本整形外科学会誌 76(8)

      Pages: 1109-1109

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] The activity of chondrocytes which were cryopreserved and the effect of hyaluronic acid and Sakamoto growth factor.2002

    • Author(s)
      Masamitsu S, Ryu J, Ishii T, Kudoh T, Mori S
    • Journal Title

      J. Jpn. Orthop. Assoc. 76(8)

      Pages: 1109-1109

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] 凍結保存した人関節軟骨細胞における細胞活性の経時的変化2001

    • Author(s)
      森聖, 龍順之助, 福島一雅, 石井隆雄, 工藤太郎, 坂本雅光
    • Journal Title

      日本整形外科学会誌 75(8)

      Pages: 967-967

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Time course change in the activity of human chondrocytes which were cryopreserved and effect of growth factor.2001

    • Author(s)
      Mori S, Ryu J, Fukushima K, Ishii T, Kudoh T, Sakamoto M
    • Journal Title

      J. Jpn. Orthop. Assoc. 75(8)

      Pages: 967-967

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 工藤太郎, 龍順之助 他: "軟骨におけるIL-1による軟骨破壊に対する抑制作用"日本整形外科学会雑誌. 77巻8号. S1136 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Masamitsu Sakamoto, et al.: "RESPONSE OF CRYOPRESERVED CHONDROCYTES TO GROWTH FACTORS"The Nihon University Journal of Medicine. Vol.45 No.2. 1-12 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 坂本雅光, 龍順之助 他: "凍結軟骨細胞におけるヒアルロン酸と成長因子の影響"日本整形外科学会雑誌. 76巻8号. S1109 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 森 聖, 龍順之助 他: "凍結保存したヒト関節軟骨細胞における細胞活性の経時的変化"日本整形外科学会雑誌. 75巻8号. S967 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kazumasa Fukushima, et al.: "The Use of Antifibrosis Agent to Improve Muscle Recovery after Laceration"American Journal of Sports Medicine. 29・4. 394-402 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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